School of Energy and Environment, Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), City University of Hong Kong, Kowloon, Hong Kong; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.
School of Energy and Environment, Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), City University of Hong Kong, Kowloon, Hong Kong; Research Centre for the Oceans and Human Health, City University of Hong Kong Shenzhen Research Institute, Shenzhen, 518057, China.
Environ Pollut. 2020 Nov;266(Pt 2):115303. doi: 10.1016/j.envpol.2020.115303. Epub 2020 Aug 14.
In this study, we determined the concentrations of Cu, Zn, Ni, Cd, Pb and As and their subcellular distributions within the tissues of mussels (Bathymodiolus marisindicus) and snails (Gigantopelta aegis) from two hydrothermal vent regions, i.e., Tiancheng and Longqi, at Southwest Indian Ridge. Mussels collected from the two venting regions showed comparable concentrations for Ni and Pb, but Cu, Zn, Cd and As concentrations were significantly different in mussel gills between the two vent regions. Similar ranges of metal concentrations were found in the snails as those in the mussels, but most of the metals were mainly accumulated in the viscera, except for Ni. Similar subcellular partitioning of Cu, Zn and Cd was documented in different mussel tissues, with cellular debris (50%) being the predominant fraction, followed by equivalent values in other fractions. Lead was distributed in both cellular debris and metal-rich granules (MRG) fraction, whereas Ni was predominantly distributed in MRG (90%). Arsenic was mainly partitioned in cellular debris and metallothionein-like protein. However, deep-sea snails displayed elevated subcellular partitioning of Cu in the organelles (up to 60%) and may be more susceptible to Cu stress than the mussels. Our results demonstrated the metal-specificity of detoxification strategies in these deep-sea hydrothermal vent mollusks, and the mussels may be more adaptable to high metal exposures than the snails at hydrothermal vent.
在这项研究中，我们测定了来自西南印度洋脊天城和龙崎两个热液喷口区贻贝（Bathymodiolus marisindicus）和蜗牛（Gigantopelta aegis）组织内 Cu、Zn、Ni、Cd、Pb 和 As 的浓度及其亚细胞分布。来自两个喷口区的贻贝表现出相似的 Ni 和 Pb 浓度，但贻贝鳃中的 Cu、Zn、Cd 和 As 浓度在两个喷口区之间有显著差异。蜗牛中的金属浓度与贻贝相似，但大多数金属主要积累在内脏中，除了 Ni。在不同的贻贝组织中，Cu、Zn 和 Cd 的亚细胞分配存在相似的范围，细胞碎片（50%）是主要部分，其他部分的含量相当。Pb 分布在细胞碎片和富含金属的颗粒（MRG）中，而 Ni 主要分布在 MRG（90%）中。As 主要分配在细胞碎片和金属硫蛋白样蛋白中。然而，深海蜗牛显示细胞器中 Cu 的亚细胞分配增加（高达 60%），可能比贻贝更容易受到 Cu 胁迫。我们的结果表明，这些深海热液喷口软体动物具有金属特异性的解毒策略，贻贝可能比蜗牛更能适应热液喷口的高金属暴露。
