Key Laboratory of UV-Emitting Materials and Technology, Ministry of Education, Northeast Normal University, Changchun, 130024, Jilin, China.
Mikrochim Acta. 2020 Aug 25;187(9):516. doi: 10.1007/s00604-020-04504-3.
Aiming to the ongoing challenge of accurate and sensitive detection for cancer biomarkers, antibody-functionalized NaYF:Yb, Er@SiO nanorods were developed as upconversion luminescence (UCL)-infrared absorption (IRA) nanoprobes. Benefiting from the shielding effect of the SiO shell, an enhanced UCL was achieved. Additionally, an IRA detection signal was introduced by the Si-O-Si bonds of SiO. Its mutual verification with UCL signal was favorable for ensuring the accuracy of the assay. A UCL-IRA sandwich detection method was established for the detection of the prostate-specific antigen. The UCL intensity at 542 nm and IRA at 1095 cm were chosen for quantitative assay. The method has high sensitivity (0.05 pg mL) and selectivity. The range of detection (200 fg mL-200 ng mL) was singnificantly broadened compared with that of single-readout UCL or IRA detection. The assay performance of human serum samples demonstrated the practicability of the method in clinical cancer diagnosis. Graphical abstract.
针对癌症生物标志物准确灵敏检测这一持续存在的挑战，研制了抗体功能化 NaYF:Yb,Er@SiO 纳米棒作为上转换发光（UCL）-红外吸收（IRA）纳米探针。得益于 SiO 壳的屏蔽效应，实现了增强的 UCL。此外，SiO 的 Si-O-Si 键引入了 IRA 检测信号。它与 UCL 信号的相互验证有利于确保分析的准确性。建立了一种用于检测前列腺特异性抗原的 UCL-IRA 夹心检测方法。选择 542nm 的 UCL 强度和 1095cm 的 IRA 进行定量分析。该方法具有高灵敏度（0.05pgmL）和选择性。与单读 UCL 或 IRA 检测相比，检测范围（200fgmL-200ngmL）显著拓宽。人血清样本的测定性能证明了该方法在临床癌症诊断中的实用性。
