rDNA 不稳定性的酵母酿酒分析。

Gel Electrophoresis Analysis of rDNA Instability in Saccharomyces cerevisiae.

机构信息

Laboratory of Genome Regeneration, Institute for Quantitative Biosciences, University of Tokyo, Tokyo, Japan.

出版信息

Methods Mol Biol. 2021;2153:403-425. doi: 10.1007/978-1-0716-0644-5_28.

Abstract

The ribosomal RNA (rDNA) sequence is the most abundant repetitive element in the budding yeast genome and forms a tandem cluster of ~100-200 copies. Cells frequently change their rDNA copy number, making rDNA the most unstable region in the budding yeast genome. The rDNA region experiences programmed replication fork arrest and subsequent formation of DNA double-strand breaks (DSBs), which are the main drivers of rDNA instability. The rDNA region offers a unique system to understand the mechanisms that respond to replication fork arrest as well as the mechanisms that regulate repeat instability. This chapter describes three methods to assess rDNA instability.

摘要

核糖体 RNA (rDNA) 序列是芽殖酵母基因组中最丰富的重复元件，形成约 100-200 个拷贝的串联簇。细胞经常改变其 rDNA 拷贝数，使 rDNA 成为芽殖酵母基因组中最不稳定的区域。rDNA 区域经历程序性复制叉停滞和随后形成 DNA 双链断裂 (DSB)，这是 rDNA 不稳定性的主要驱动因素。rDNA 区域提供了一个独特的系统来了解响应复制叉停滞的机制以及调节重复不稳定性的机制。本章描述了三种评估 rDNA 不稳定性的方法。