阿格列苷 I 抑制牙龈卟啉单胞菌 TDC60 的致病性。

Argeloside I inhibits the pathogenicity of Porphyromonas gingivalis TDC60.

机构信息

The United Graduate School of Agricultural Sciences, Tottori University, 4-101 Koyama-Minami, Tottori 680-8553, Japan.

Arid Land Research Center, Tottori University, 1390 Hamasaka, Tottori 680-0001, Japan; Department of Food Science and Technology, Faculty of Agriculture, University of Khartoum, Shambat, Khartoum North 14413, Sudan.

出版信息

J Biosci Bioeng. 2020 Dec;130(6):644-649. doi: 10.1016/j.jbiosc.2020.07.016. Epub 2020 Aug 23.

DOI:10.1016/j.jbiosc.2020.07.016

PMID:32847740

Abstract

Porphyromonas gingivalis, a major pathogen associated with chronic periodontitis, produces several virulence agents in the outer cell membrane, including gingipains and hemagglutinins. These virulence factors enable the bacteria to adhere to periodontal tissue and degrade host proteins to obtain the nutrients needed for dental plaque formation. P. gingivalis TDC60 was recently identified as the most aggressive P. gingivalis strain to dates. In this study, we isolated a known pregnane glycoside, argeloside I, from the aqueous extract of Solenostemma argel leaves. Argeloside I completely hindered the growth of P. gingivalis TDC60 and inhibited the production of hemagglutinins as well as Arg- and Lys-specific gingipains. Our results demonstrate a new function of pregnane glycosides. Argeloside I may be a candidate for reducing the risk associated with P. gingivalis TDC60 and its adhesion factors.

摘要

牙龈卟啉单胞菌是与慢性牙周炎相关的主要病原体，在外膜中产生几种毒力因子，包括牙龈蛋白酶和血凝结素。这些毒力因子使细菌能够附着在牙周组织上，并降解宿主蛋白以获取牙菌斑形成所需的营养物质。最近，牙龈卟啉单胞菌 TDC60 被确定为迄今为止最具侵袭性的牙龈卟啉单胞菌菌株。在这项研究中，我们从萝藦属植物的水提取物中分离出一种已知的孕烷糖苷，即阿格尔糖苷 I。阿格尔糖苷 I 完全抑制了牙龈卟啉单胞菌 TDC60 的生长，并抑制了血凝结素以及 Arg 和 Lys 特异性牙龈蛋白酶的产生。我们的结果表明孕烷糖苷具有新的功能。阿格尔糖苷 I 可能是降低与牙龈卟啉单胞菌 TDC60 及其粘附因子相关风险的候选药物。