Yu Hailong, Song Lilong, Cao Xiang, Li Wei, Zhao Yuanyuan, Chen Jian, Li Jun, Chen Yingzhu, Yu Wenkui, Xu Yun
Affiliated of Drum Tower Hospital, Medical School of Nanjing University, Nanjing, China.
Clinical Medical College of Yangzhou University, Yangzhou, China.
Front Pharmacol. 2020 Jul 30;11:1173. doi: 10.3389/fphar.2020.01173. eCollection 2020.
Cerebral ischaemia/reperfusion (CI/R) injury is a major challenge due to the lack of effective neuroprotective drugs. Hederagenin (HE) is the aglycone part of saponins extracted from that has exhibited anti-apoptotic and anti-inflammatory effects; however, the role of HE in CI/R has not been elucidated. In this study, mice were intraperitoneally (i.p.) injected with HE (26.5, 53, or 106 μmol/kg body weight) for 3 days after middle cerebral artery occlusion (MCAO). Neural function and brain infarct volume were evaluated. HE treatment attenuated CI/R-induced apoptosis and inflammatory cytokine expression within the infarcted areas. HE treatment also decreased the activation of the MLK3 signalling pathway, which potentiates CI/R damage the MAPK and NFκB pathways. Due to HE's safety profile, it has potential to be used for the clinical treatment of ischaemic stroke.
由于缺乏有效的神经保护药物，脑缺血/再灌注（CI/R）损伤是一个重大挑战。常春藤皂苷元（HE）是从[植物名称未给出]中提取的皂苷的苷元部分，已表现出抗凋亡和抗炎作用；然而，HE在CI/R中的作用尚未阐明。在本研究中，小鼠在大脑中动脉闭塞（MCAO）后腹腔注射HE（26.5、53或106 μmol/kg体重），持续3天。评估神经功能和脑梗死体积。HE治疗减轻了CI/R诱导的梗死区域内的细胞凋亡和炎性细胞因子表达。HE治疗还降低了MLK3信号通路的激活，该通路通过MAPK和NFκB通路增强CI/R损伤。由于HE的安全性，它有潜力用于缺血性中风的临床治疗。
