Al-Mogairen Sultan
Department of Medicine, Division of Rheumatology, King Saud University, Riyadh, Saudi Arabia.
Arch Rheumatol. 2020 Feb 7;35(2):220-225. doi: 10.46497/ArchRheumatol.2020.7164. eCollection 2020 Jun.
This study aims to demonstrate the effect of subcutaneous injections of abatacept on the histology of testes in mice.
The study included 20 male BALB/c mice (average weight, 25 g; aged 12-14 weeks). Ten mice received subcutaneous (SC) injections of abatacept [0.25 mg per 25 g body weight per 0.03 mL normal saline (NS)] at zero, two, four and eight weeks. As the control group, 10 mice received SC injections of NS (0.03 mL). At the post-injection 10 week, the mice were sacrificed, and histopathological studies were conducted.
The results showed that 3/10 mice died of the abatacept-treated group. Testicular histology for the abatacept-treated group showed that 7/7 displayed no histopathological changes.
To our knowledge, this is the first control-blinded study of BALB/c mice suggesting that abatacept may not have testicular toxicity. Further fertility and testicular toxicology evaluations including semen analysis and gonadal hormones should be performed to clarify our findings.
本研究旨在证明皮下注射阿巴西普对小鼠睾丸组织学的影响。
该研究纳入20只雄性BALB/c小鼠（平均体重25 g；年龄12 - 14周）。10只小鼠在第0、2、4和8周接受皮下注射阿巴西普[每25 g体重0.25 mg阿巴西普溶于0.03 mL生理盐水（NS）]。作为对照组，10只小鼠接受皮下注射生理盐水（0.03 mL）。在注射后第10周，处死小鼠并进行组织病理学研究。
结果显示，阿巴西普治疗组中有3/10的小鼠死亡。阿巴西普治疗组的睾丸组织学检查显示，7/7未出现组织病理学变化。
据我们所知，这是第一项针对BALB/c小鼠的对照盲法研究，表明阿巴西普可能没有睾丸毒性。应进行进一步的生育力和睾丸毒理学评估，包括精液分析和性腺激素检测，以阐明我们的研究结果。
