School of Pharmaceutical Science (Shenzhen), Sun Yat-sen University, Guangzhou, China.
Department of Anatomy and Convergence Medical Science, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju, Republic of Korea.
J Sci Food Agric. 2021 Mar 15;101(4):1499-1506. doi: 10.1002/jsfa.10763. Epub 2020 Sep 11.
With an increasing aging population, postmenopausal osteoporosis has become a global public health problem. Previous evidence has shown that postmenopausal osteoporosis is a skeletal disease mainly caused by estrogen deficiency, generally accompanied by inflammation, and dietary isoflavones may ameliorate postmenopausal osteoporosis by anti-inflammatory activity. We have generated isoflavone-enriched soybean leaves (IESLs), but their anti-inflammatory activity and effect on attenuating osteoporosis are still obscure. Here, we determined the isoflavone profiles of IESLs and evaluated their anti-inflammatory activity in lipopolysaccharide-stimulated RAW 264.7 cells and anti-osteoporotic effects on ovariectomy-induced osteoporosis in rats.
IESLs had a high content of total isoflavone. Hydrolysate of IESLs (HIESLs) was rich with the aglycones daidzein and genistein, and HIESLs can significantly inhibit lipopolysaccharide-induced inflammation by reducing messenger RNA expression of iNOS, COX-2, IL6, and IL1β. Moreover, ovariectomized rats receiving aqueous extracts of IESLs (HIESLs) orally maintained more bone mass than control rats did, which was attributed to inhibition of osteoclastogenesis by downregulating the messenger RNA expression of the bone-specific genes RANKL/OPG, OC, and cathepsin K, and the inflammation-related genes IL6, NFκB, and COX-2.
IESLs may attenuate postmenopausal osteoporosis by suppressing osteoclastogenesis with anti-inflammatory activity and be a potential source of functional food ingredients for the prevention of osteoporosis. © 2020 Society of Chemical Industry.
随着人口老龄化的增加，绝经后骨质疏松症已成为全球性公共卫生问题。先前的证据表明，绝经后骨质疏松症是一种主要由雌激素缺乏引起的骨骼疾病，通常伴有炎症，而膳食异黄酮可能通过抗炎活性改善绝经后骨质疏松症。我们已经产生了富含异黄酮的大豆叶（IESLs），但它们的抗炎活性和减轻骨质疏松症的效果仍不清楚。在这里，我们确定了 IESLs 的异黄酮谱，并评估了它们在脂多糖刺激的 RAW 264.7 细胞中的抗炎活性以及对去卵巢诱导的骨质疏松症大鼠的抗骨质疏松作用。
IESLs 具有很高的总异黄酮含量。IESLs 的水解产物（HIESLs）富含苷元大豆苷元和染料木黄酮，并且 HIESLs 可以通过降低 iNOS、COX-2、IL6 和 IL1β 的信使 RNA 表达来显著抑制脂多糖诱导的炎症。此外，口服接受 IESLs 水提物（HIESLs）的去卵巢大鼠比对照大鼠保留更多的骨量，这归因于通过下调骨特异性基因 RANKL/OPG、OC 和组织蛋白酶 K 的信使 RNA 表达以及炎症相关基因 IL6、NFκB 和 COX-2 抑制破骨细胞生成。
IESLs 可能通过抗炎活性抑制破骨细胞生成来减轻绝经后骨质疏松症，并且可能成为预防骨质疏松症的功能性食品成分的潜在来源。© 2020 化学工业学会。
