Department Ⅱ of Endocrinology, Handan Central Hospital, Handan, China.
Department Ⅱ of Endocrinology, Third Affiliated Hospital of Hebei Medical University, Shijiazhuang, China.
J Clin Lab Anal. 2020 Dec;34(12):e23531. doi: 10.1002/jcla.23531. Epub 2020 Aug 26.
Papillary thyroid cancer (PTC) is a kind of thyroid cancer. Previous studies showed that insulin-like growth factor-1 (IGF1) plays an important role in tumorigenesis, development, invasion, and metastasis. However, the function of IGF1 in PTC progression remains unclear.
Seventy-three pairs of PTC tissue specimens and adjacent normal specimens form and normal cell line and PTC cell lines were collected in this study. The immunohistochemistry (IHC) assay was performed to test the expression of IGF1. The RNA isolation and quantitative real-time PCR assays (qRT-PCR assays) and Western blot analysis were used to test mRNA and protein expression. Cell proliferation assay, EdU assay, flow cytometry assay, wound healing assay, and Transwell invasion assay were performed to test cell proliferation, invasion, and apoptosis.
We found that the expression of IGF1 in PTC tissue samples was higher than that in adjacent normal specimens and was significantly associated with tumor size, TNM staging, and lymph node metastasis. Furthermore, IGF1 treatment significantly increased cell viability in a dose-dependent manner. EdU assay also demonstrated the effect of IGF1 on the proliferation of BCPAP and TPC1 cells. Moreover, IGF1 treatment effectively increased the invasive capacity of BCPAP and TPC1 cells. More importantly, IGF1 treatment could significantly enhance the phosphorylation of STAT3 in BCPAP and TPC1 cells. Moreover, cryptotanshinone (Cryp) treatment reversed the effect of IGF1 treatment on cell viability and invasion of BCPAP and TPC1 cells.
Collectively, IGF1 promotes proliferation and invasion of PTC progression through the STAT3 signaling pathway.
甲状腺癌（PTC）是一种甲状腺癌。之前的研究表明，胰岛素样生长因子-1（IGF1）在肿瘤发生、发展、侵袭和转移中起重要作用。然而，IGF1 在 PTC 进展中的作用尚不清楚。
本研究收集了 73 对 PTC 组织标本和相邻正常标本、正常细胞系和 PTC 细胞系。通过免疫组织化学（IHC）检测 IGF1 的表达。通过 RNA 分离和实时定量 PCR（qRT-PCR）和 Western blot 分析检测 mRNA 和蛋白表达。通过细胞增殖试验、EdU 试验、流式细胞术试验、划痕愈合试验和 Transwell 侵袭试验检测细胞增殖、侵袭和凋亡。
我们发现 IGF1 在 PTC 组织样本中的表达高于相邻正常样本，并且与肿瘤大小、TNM 分期和淋巴结转移显著相关。此外，IGF1 处理以剂量依赖性方式显著增加细胞活力。EdU 试验还证明了 IGF1 对 BCPAP 和 TPC1 细胞增殖的影响。此外，IGF1 处理有效增加了 BCPAP 和 TPC1 细胞的侵袭能力。更重要的是，IGF1 处理可显著增强 BCPAP 和 TPC1 细胞中 STAT3 的磷酸化。此外，隐丹参酮（Cryp）处理逆转了 IGF1 处理对 BCPAP 和 TPC1 细胞活力和侵袭的影响。
总之，IGF1 通过 STAT3 信号通路促进 PTC 进展中的增殖和侵袭。
