Department of Chemical and Biomolecular Engineering, and Institute for NanoBioTechnology, The Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, United States.
Biologics Process Development, Global Product Development and Supply, Bristol-Myers Squibb, Devens, Massachusetts 01434, United States.
Nano Lett. 2020 Oct 14;20(10):6957-6965. doi: 10.1021/acs.nanolett.0c01297. Epub 2020 Sep 3.
The separation and purification of therapeutic proteins from their biological resources pose a great limitation for industrial manufacturing of biologics in an efficient and cost-effective manner. We report here a supramolecular polymeric system that can undergo multiple reversible processes for efficient capture, precipitation, and recovery of monoclonal antibodies (mAbs). These supramolecular polymers, namely immunofibers (IFs), are formed by coassembly of a mAb-binding peptide amphiphile with a rationally designed filler molecule of varying stoichiometric ratios. Under the optimized conditions, IFs can specifically capture mAbs with a precipitation yield greater than 99%, leading to an overall mAb recovery yield of 94%. We also demonstrated the feasibility of capturing and recovering two mAbs from clarified cell culture harvest. These results showcase the promising potential of peptide-based supramolecular polymers as reversible affinity precipitants for mAb purification.
从生物资源中分离和纯化治疗性蛋白质，这对生物制品的高效和经济有效地工业制造构成了极大的限制。我们在这里报告了一种超分子聚合物系统，该系统可以进行多种可逆过程，从而有效地捕获、沉淀和回收单克隆抗体 (mAb)。这些超分子聚合物，即免疫纤维 (IF)，是由与具有不同化学计量比的合理设计的填充分子共聚组装而成的 mAb 结合肽两亲物形成的。在优化条件下，IF 可以特异性地捕获沉淀产率大于 99%的 mAb，导致整体 mAb 回收率为 94%。我们还证明了从澄清的细胞培养物收获物中捕获和回收两种 mAb 的可行性。这些结果展示了基于肽的超分子聚合物作为 mAb 纯化的可逆亲和沉淀剂的有前途的潜力。
