Naldoni Juliana, Carriero Mateus M, Moreira Gabriel S A, da Silva Marcia R M, Maia Antonio A M, Adriano Edson A
Department of Ecology and Evolutionary Biology, Federal University of São Paulo (UNIFESP), Diadema, SP, Brazil.
Department of Animal Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil.
Parasitol Res. 2020 Nov;119(11):3627-3637. doi: 10.1007/s00436-020-06863-5. Epub 2020 Aug 27.
This study increases the known biodiversity of cnidarian parasites in neotropical bryconid fishes. Two novel Myxobolus species are described based on morphology, ultrastructure and small subunit ribosomal DNA (ssrDNA) sequencing: Myxobolus vetuschicanus n. sp. infecting fins of Salminus franciscanus and Myxobolus mineirus n. sp. infecting the mesentery of Brycon orthotaenia from the São Francisco River basin, Minas Gerais State, Brazil. Ultrastructural analysis of the two species revealed an asynchronous sporogenesis process, with germinative cells and young developmental stages of myxospores in the periphery of the plasmodia. In M. vetuschicanus n. sp., the plasmodia were surrounded by a layer of fibroblasts and in M. mineirus n. sp., the plasmodial membrane had direct contact with the host tissue. The phylogenetic analysis based on the ssrDNA of Henneguya/Myxobolus species showed that the two novel Myxobolus species grouped in subclades together with other parasite species of bryconid fishes.
本研究增加了新热带区脂鲤科鱼类刺胞动物寄生虫的已知生物多样性。基于形态学、超微结构和小亚基核糖体DNA（ssrDNA）测序，描述了两个新的粘孢子虫物种：感染巴西米纳斯吉拉斯州圣弗朗西斯科河流域弗朗西斯克脂鲤鳍部的维图希卡粘孢子虫（Myxobolus vetuschicanus n. sp.）和感染直带脂鲤肠系膜的米内鲁斯粘孢子虫（Myxobolus mineirus n. sp.）。对这两个物种的超微结构分析显示出孢子发生过程不同步，在原质团周边有胚细胞和粘孢子的幼体发育阶段。在维图希卡粘孢子虫中，原质团被一层成纤维细胞包围，而在米内鲁斯粘孢子虫中，原质团膜与宿主组织直接接触。基于亨内古亚属/粘孢子虫属物种ssrDNA的系统发育分析表明，这两个新的粘孢子虫物种与脂鲤科鱼类的其他寄生虫物种一起聚集在亚分支中。
