Capodifoglio Kassia R H, Adriano Edson A, Milanin Tiago, Silva Marcia R M, Maia Antônio A M
Departamento de Medicina Veterinária, Faculdade de Zootecnia e Engenharia de Alimentos, Universidade de São Paulo (USP), Rua Duque de Caxias Norte, 225, CEP, Pirassununga, SP 13635-900, Brazil.
Departamento de Ciências Biológicas, Universidade Federal de São Paulo (UNIFESP), Rua Professor Artur Riedel, 275, Jardim Eldorado, CEP, Diadema, SP 09972-270, Brazil; Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Caixa Postal 6109, CEP, Campinas, SP 13083-970, Brazil.
Parasitol Int. 2016 Jun;65(3):184-90. doi: 10.1016/j.parint.2015.12.006. Epub 2015 Dec 17.
Myxobolus hilarii n. sp. was described, based on morphology, histology, ultrastructure and 18S rDNA sequencing, infecting the kidney of Brycon hilarii (Valenciennes 1850) (Characiformes: Bryconidae) taken from fish farms in the state of São Paulo, Brazil. Thirteen specimens of B. hilarii were examined and 100% had round, white plasmodia in the kidney. The mature myxospores were rounded, measuring 11.5 ± 0.8 (9.8-13.4) μm in length, 11.0 ± 0.7 (9.7-12.4) μm in width and 7.6 ± 1.0 (6.7-9.0) μm in thickness. Polar capsules were elongated and of equal size, with 6.5 ± 0.4 (6.0-7.2) μm in length and 4.0 ± 0.2 (3.6-5.3) μm in width and their polar filaments had 5 to 7 coils. Histological analysis revealed plasmodial development in the renal tubules, causing compression and deformation of adjacent tissues and destruction of renal tubule cells. Ultrastructural analysis showed direct contact between the plasmodial wall and the host tissue and asynchronous plasmodial development. The phylogenetic analysis of South American myxobolids, based on 18S rDNA sequencing, showed the myxosporeans grouping into two main clades. M. hilarii n. sp. appears as sister species of Myxobolus piraputangae.
基于形态学、组织学、超微结构和18S rDNA测序，描述了新种希拉里碘泡虫（Myxobolus hilarii n. sp.），它感染了取自巴西圣保罗州养鱼场的希氏魮脂鲤（Brycon hilarii，瓦朗谢讷，1850年）（脂鲤目：魮脂鲤科）的肾脏。检查了13尾希氏魮脂鲤标本，100%的鱼肾脏中有圆形白色的孢囊。成熟的粘孢子呈圆形，长11.5±0.8（9.8 - 13.4）μm，宽11.0±0.7（9.7 - 12.4）μm，厚7.6±1.0（6.7 - 9.0）μm。极囊细长且大小相等，长6.5±0.4（6.0 - 7.2）μm，宽4.0±0.2（3.6 - 5.3）μm，其极丝有5至7圈。组织学分析显示肾小管中有孢囊发育，导致相邻组织受压和变形以及肾小管细胞破坏。超微结构分析显示孢囊壁与宿主组织直接接触以及孢囊发育不同步。基于18S rDNA测序对南美碘泡虫进行的系统发育分析表明，粘孢子虫分为两个主要分支。希拉里碘泡虫新种表现为皮拉普坦碘泡虫（Myxobolus piraputangae）的姐妹种。
