ICP-MS 法结合多元数据分析 EDTA 螯合疗法期间尿液元素排泄谱：病例研究。
Determination by ICP-MS and multivariate data analysis of elemental urine excretion profile during the EDTA chelation therapy: A case study.
机构信息
Department of Sciences and Technological Innovation, University of Piemonte Orientale, Viale Michel 11, 15121, Alessandria, Italy.
Department of Translational Medicine, University of Piemonte Orientale, Via Solaroli 17, 28100 Novara, Italy; Isalit srl, c/o IPAZIA, Corso Trieste 15/A, 28100 Novara, Italy.
出版信息
J Trace Elem Med Biol. 2020 Dec;62:126608. doi: 10.1016/j.jtemb.2020.126608. Epub 2020 Aug 14.
BACKGROUND
Based on the medical history and laboratory analytical tests, a patient presenting symptoms compatible with Chronic Fatigue Syndrome was suspected of metal intoxication; therefore, a chelating therapy was attempted. In parallel, the profile of elemental excretion in urine was determined.
METHODS
Chelation therapy by CaNaEDTA was administered every two weeks and urine samples were routinely collected for 17 months. The samples were mineralized with HNO 69 % and analyzed by Inductively-Coupled Plasma - Mass Spectrometry. Data were processed by multivariate statistical methods.
RESULTS
Most of the toxic elements showed a peak of excretion in 12-24 h after EDTA administration, which returned to basal level by 24-36 h after the treatment. Yet, the excretion of some trace elements persisted in the urine collected 26 h after the treatment.
CONCLUSIONS
The analysis of excreted metals following the CaNaEDTA infusion allowed to monitor dynamically the chelation therapy. The chelation therapy was effective in mobilizing and eliminating the principal heavy metals present from the body. However, since such clearance almost vanished 24 h after the treatment, a protocol with more frequent and low-dose administrations is advisable to improve the metal excretion.
背景
根据病史和实验室分析检测结果，一位出现疑似慢性疲劳综合征症状的患者疑似金属中毒，因此尝试了螯合疗法。同时，还确定了尿液中元素排泄情况。
方法
每两周通过 CaNaEDTA 进行螯合疗法，并常规收集尿液样本 17 个月。将样本用 69%的 HNO 3 矿化，并用电感耦合等离子体质谱法进行分析。数据通过多元统计方法进行处理。
结果
大多数有毒元素在 EDTA 给药后 12-24 小时达到排泄峰值，在治疗后 24-36 小时恢复到基础水平。然而，一些微量元素的排泄在治疗后 26 小时收集的尿液中仍然存在。
结论
通过 CaNaEDTA 输注后分析排泄的金属，可以动态监测螯合疗法。螯合疗法有效动员并从体内清除了主要存在的重金属。然而，由于治疗后 24 小时几乎清除了这种清除作用，因此建议采用更频繁和低剂量的给药方案以改善金属排泄。
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