College of Bio-systems Engineering and Food Science, Zhejiang University, Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Hangzhou, 310058, China; Department of Agricultural and Environmental Engineering, Faculty of Engineering, Bayero University, Kano, Nigeria.
College of Bio-systems Engineering and Food Science, Zhejiang University, Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture, Ministry of Agriculture, Hangzhou, 310058, China.
J Environ Manage. 2020 Dec 1;275:111264. doi: 10.1016/j.jenvman.2020.111264. Epub 2020 Aug 24.
In this study, a novel sponge biocarriers (SB) in moving bed bioreactor (MBBR) treating recirculating aquaculture systems wastewater was evaluated for the first time. Two lab-scale MBBRs were operated simultaneously for 116 days under various hydraulic retention times (HRTs). The reactors R1 and R2 were filled with K5 plastic carriers and SB, respectively. From the results, at an optimum HRT of 6 h, ammonia removal efficiency and nitrification rate were 86.67 ± 2.4% and 1.43 mg/L.h for the R1 and, 91.65 ± 1.3% and 1.52 mg/L.h for the R2, respectively. The microbial community analysis showed that the predominant genera in the nitrifying community were Nitrosomonas (AOB) and Nitrospira (NOB) in co-existence with heterotrophic genera Hyphomicrobium, Mesorhizobium, Zhizhongheella, and Klebsiella spp. Modified Stover-Kincannon model examined the ammonia removal kinetics, and the values of kinetic parameters obtained were U: 0.909 and 1.111 g/L.d and K: 0.929 and, 1.108 g/L.d for the R1 and R2, respectively. The correlation coefficients (R) of the MBBRs were higher than 0.98, indicating that the model adequately described the experimental data. Overall, MBBR, filled with the proposed novel SB operated at 6 h HRT, can achieve the highest nitrification performance and increase the diversity of the functional microbial communities.
本研究首次评估了新型海绵生物载体（SB）在移动床生物反应器（MBBR）中处理循环水产养殖系统废水的效果。两个实验室规模的 MBBR 同时运行了 116 天，水力停留时间（HRT）不同。反应器 R1 和 R2 分别填充 K5 塑料载体和 SB。结果表明，在最佳 HRT 为 6 h 时，R1 的氨去除效率和硝化速率分别为 86.67±2.4%和 1.43 mg/L.h，R2 分别为 91.65±1.3%和 1.52 mg/L.h。微生物群落分析表明，硝化群落中的优势属为亚硝化单胞菌（AOB）和硝化螺旋菌（NOB），同时存在异养属嗜氢菌属、中慢生根瘤菌属、志仲贺菌属和克雷伯氏菌属。修正的Stover-Kincannon 模型检验了氨去除动力学，得到的动力学参数值 U 为 0.909 和 1.111 g/L.d，K 为 0.929 和 1.108 g/L.d，分别为 R1 和 R2。MBBR 的相关系数（R）均高于 0.98，表明该模型能够很好地描述实验数据。总体而言，在 6 h HRT 下运行的填充有新型 SB 的 MBBR 可以实现最高的硝化性能，并增加功能微生物群落的多样性。
