Cariology Unit, Department of Oral Rehabilitation and Health Sciences Faculty, School of Dentistry, University of Talca, 1 Poniente 1141, Talca, Chile.
Preventive and Community Dentistry Department, Dental School, Federal University of Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos 2492, Porto Alegre, Brazil.
Arch Oral Biol. 2020 Oct;118:104878. doi: 10.1016/j.archoralbio.2020.104878. Epub 2020 Aug 8.
This study aimed to evaluate the anti-caries effect of a fluoridated milk-based drink on a root caries model by assessing mineral loss and both biofilm microbial viability and acidogenicity under increasing concentrations of fluoride supplementation.
Streptococcus mutans UA159 biofilms were grown on root dentin slabs for five days. The slabs were randomly assigned to following groups: milk-based drink (G1) and milk-based drink supplemented with 5-ppm NaF (G2), 10 ppm NaF (G3), and 20 ppm NaF (G4). A 10% sucrose and 0.9% NaCl solution were used as positive and negative-caries controls, respectively. Slabs/biofilms were exposed to the different treatments 3 times/day for 5 min. To estimate biofilm acidogenicity, the pH of the spent media was serially measured to calculate the area above the curve. Viable bacteria and dentin demineralization were assessed after the experimental phase. Results were compared using ANOVA followed by the Tukey test.
G1 exhibited slightly lower acidogenicity than the positive caries control group (p < 0.05). G2, G3, and G4 induced lower acidogenicity than 10% sucrose and the non-supplemented milk-based drink. The lowest acidogenicity was found in G4 (p < 0.05). Fluoride-supplemented milk-based drinks (G2, G3, and G4) resulted in lower bacterial counts (p < 0.05) and induced lower demineralization (p < 0.05) than the positive caries control and non-supplemented milk-beverage (G1). There was a dose-dependent inhibition of demineralization with fluoride-supplemented milk-based drinks.
Fluoride supplementation of a milk-based drink for older adults may reduce its cariogenicity in root dentin.
本研究旨在通过评估根龋模型中氟化牛奶饮料的抗龋效果，来评估在氟化物补充浓度增加的情况下，矿质损失以及生物膜微生物活力和产酸情况。
将变异链球菌 UA159 生物膜在根牙本质板上培养五天。将牙本质板随机分配到以下组：牛奶饮料（G1）和添加 5ppm NaF 的牛奶饮料（G2）、10ppm NaF（G3）和 20ppm NaF（G4）。10%蔗糖和 0.9%NaCl溶液分别作为阳性和阴性对照。牙本质板/生物膜每天暴露于不同处理 3 次，每次 5 分钟。为了评估生物膜产酸能力，连续测量消耗培养基的 pH 值以计算曲线下的面积。实验结束后评估活细菌和牙本质脱矿情况。结果使用方差分析（ANOVA）后进行 Tukey 检验。
G1 的产酸能力略低于阳性龋对照组（p<0.05）。G2、G3 和 G4 的产酸能力低于 10%蔗糖和未添加的牛奶饮料。G4 的产酸能力最低（p<0.05）。添加氟化物的牛奶饮料（G2、G3 和 G4）的细菌计数较低（p<0.05），且脱矿程度低于阳性龋对照组和未添加的牛奶饮料（G1）（p<0.05）。添加氟化物的牛奶饮料对脱矿的抑制作用呈剂量依赖性。
在老年人的牛奶饮料中添加氟化物可能会降低其在根牙本质中的致龋性。
