Zhejiang Provincial Key Lab of Equipment Electronics, Hangzhou Dianzi University, Hangzhou 310018, PR China; School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, PR China.
School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, PR China.
Micron. 2020 Nov;138:102920. doi: 10.1016/j.micron.2020.102920. Epub 2020 Aug 15.
Considering angle diversity and synthetic aperture, Fourier ptychographic microscopy (FPM) could address contradiction of high resolution and wide field of view. However, in the conventional FPM method, large capture quantity leads to poor efficiency. So, an efficient FPM method based on optimized pattern of LED angle illumination is proposed. Firstly, from position relationship between the LED, aperture and sample, we obtain the theoretical expandable spectrum range and the spectrum distribution in Fourier space of all LEDs. Secondly, we u se image quality assessment methods to extract differential expressions between an arbitrary LED illumination and full LEDs illumination. Thirdly, the optimized angle illumination strategy is achieved according to the analysis of different expressions. Based on this method, we design a rhombus-based illumination method for the LED array to accelerate the FPM efficiency. Finally, we validate the effectiveness and efficiency of our method with both simulated and real experiments. The results indicate that our method can effectively improve the efficiency for FPM without sacrificing image reconstruction quality.
考虑角度多样性和合成孔径，傅里叶叠层显微镜（FPM）可以解决高分辨率和大视场的矛盾。然而，在传统的 FPM 方法中，大量的捕获量导致效率低下。因此，提出了一种基于优化 LED 角度照明模式的高效 FPM 方法。首先，从 LED、孔径和样品之间的位置关系出发，得到了所有 LED 的理论扩展谱范围和傅里叶空间中的谱分布。其次，使用图像质量评估方法提取任意 LED 照明和全 LED 照明之间的差分表达式。然后，根据不同表达式的分析，得到优化的角度照明策略。基于此方法，我们为 LED 阵列设计了一种基于菱形的照明方法，以提高 FPM 的效率。最后，我们通过模拟和实际实验验证了我们的方法的有效性和效率。结果表明，我们的方法可以在不牺牲图像重建质量的情况下，有效地提高 FPM 的效率。
