Van Training and Research Hospital, Ophthalmology Clinic, University of Health Sciences, Van, Turkey.
Engineering Faculty, Van Yuzuncu Yil University, Van, Turkey.
Curr Eye Res. 2021 Apr;46(4):490-495. doi: 10.1080/02713683.2020.1812088. Epub 2020 Aug 28.
: The aim of this study is to show the pseudoexfoliation syndrome (PES) material accumulated in the lens anterior capsule and the trace elements directly with using scanning electron microscopy (SEM) and to investigate the effect of PES materials on aqueous humor by chemical analyses.: The study groups consisted of 30 patients with PES and 30 patients without PES. 0.1-0.3 mL of aqueous humor was aspirated from anterior chamber and anterior capsule sample was obtained by applying continuous curvilinear capsulorhexis. The level of selected elements (Zn, Cu, Se, Fe, Si, Al, Ca, K) were determined from aqueous humor samples via Inductively coupled plasma - optical emission spectrometry (ICP-OES) machine. The morphology and elemental distribution of anterior lens capsules were carried out via a Zeiss Sigma 300 scanning electron microscope with energy dispersive X-ray spectroscopy (semiquantitative EDX).: For aqueous humor, the ICP-OES results show that Cu and Zn levels is higher ( < .001, both of them) in PES group than that the obtained from control group. It was noticed that the ratio of Cu/Zn was higher ( = .008) in aqueous humor, while Cu/Se ratio was higher in both aqueous humor ( < .001) and anterior capsule ( < .001) for PES group. The SEM images show that different metal precipitates were obtained from anterior lens capsules with PES. However, there is no obvious element precipitates on the anterior lens capsules without PES.: The results observed that the level of selected elements can be obtained at different level in both PES group and control group. Especially, if the oxidative behavior of these elements which is thought to play a role in PES formation can be clarified, new approaches will be done to evaluate the structure of PES.
: 本研究旨在通过扫描电子显微镜（SEM）直接显示积聚在前晶状体囊中的假性剥脱综合征（PES）物质和痕量元素，并通过化学分析研究 PES 物质对房水的影响。: 研究组包括 30 名 PES 患者和 30 名无 PES 患者。从前房抽吸 0.1-0.3 毫升房水，并通过连续曲线囊切开术获得前囊样本。通过电感耦合等离子体 - 光学发射光谱仪（ICP-OES）机从房水样本中测定选定元素（Zn、Cu、Se、Fe、Si、Al、Ca、K）的水平。使用 Zeiss Sigma 300 扫描电子显微镜和能量色散 X 射线光谱仪（半定量 EDX）对前晶状体囊的形态和元素分布进行研究。: 对于房水，ICP-OES 结果表明，PES 组的 Cu 和 Zn 水平较高（<0.001，两者均如此）。注意到，PES 组房水中 Cu/Zn 比值较高（=0.008），而 Cu/Se 比值在房水（<0.001）和前囊（<0.001）中均较高。SEM 图像显示，从患有 PES 的前晶状体囊中获得了不同的金属沉淀物。然而，在没有 PES 的前晶状体囊中没有明显的元素沉淀物。: 观察到的结果表明，在 PES 组和对照组中，可以在不同水平上获得所选元素的水平。特别是，如果可以阐明这些元素的氧化行为（被认为在 PES 形成中起作用），那么就可以采取新的方法来评估 PES 的结构。
