Department of Lung Cancer, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin's Clinical Research Center for Cancer, Tianjin Lung Cancer Center, Tianjin, China.
Thorac Cancer. 2020 Oct;11(10):2887-2895. doi: 10.1111/1759-7714.13625. Epub 2020 Aug 27.
This study aimed to identify an efficient, simple, and specific method of detecting mutations in the epidermal growth factor receptor (EGFR) gene in isolated lung cancer circulating tumor cells (CTCs) and to improve the ability to obtain tumor tissue clinically.
EGFR peptide lipid magnetic spheres (EG-P-LMB) were prepared by reverse evaporation, and characterization and cell capture efficiency assessed. The peripheral blood samples of 30 lung cancer patients were isolated and identified with the EG-P-LMB using 20 healthy volunteers as controls. Finally, the isolated CTCs were tested for EGFR gene mutations, and the tissue samples selected for comparison.
The prepared magnetic spheres had a smaller particle size and higher stability according to the particle size potential test. Their morphology was homogeneous by atomic force observation, and the UV test showed that there were peptides on the surface. The separation efficiency of EG-P-LMB was greater than 90% in PBS and greater than 80% in the blood simulation system. Compared with the tissue sample results, the positive rate of EGFR gene mutations was 94%. The CTC test results of 27 patients were consistent with the tissue test results of the corresponding patients, and the consistency with the tissue comparison test results was 90% (27/30).
EG-P-LMB can effectively capture CTCs in the peripheral blood of patients with lung cancer. CTC detection can accurately identify mutations in the EGFR gene and improve the ability to obtain tumor tissue in clinical practice.
SIGNIFICANT FINDINGS OF THE STUDY: EG-P-LMB can effectively capture CTCs in the peripheral blood of patients with lung cancer. CTC detection can accurately identify mutations in the EGFR gene and improve the ability to obtain tumor tissue in clinical practice.
This study added EGFR peptide lipid magnetic spheres to capture CTCs in the blood. Genetic testing was performed and compared with tissues. It solves the problem of clinically difficult tumor tissue sampling.
本研究旨在寻找一种有效、简单、特异的方法，从分离的肺癌循环肿瘤细胞（CTC）中检测表皮生长因子受体（EGFR）基因突变，提高临床获取肿瘤组织的能力。
采用逆向蒸发法制备 EGFR 肽脂质磁珠（EG-P-LMB），并对其进行表征和细胞捕获效率评估。以 20 例健康志愿者为对照，对 30 例肺癌患者的外周血样本进行分离和 EG-P-LMB 鉴定。最后，对分离出的 CTC 进行 EGFR 基因突变检测，并与选择的组织样本进行比较。
通过粒径电位测试，所制备的磁性微球粒径较小且稳定性较高。原子力观察显示其形态均匀，紫外测试表明表面有肽。在 PBS 中，EG-P-LMB 的分离效率大于 90%；在血液模拟系统中，分离效率大于 80%。与组织样本结果相比，EGFR 基因突变的阳性率为 94%。27 例患者的 CTC 检测结果与相应患者的组织检测结果一致，与组织对比检测结果的一致性为 90%（27/30）。
EG-P-LMB 可有效捕获肺癌患者外周血中的 CTC。CTC 检测可准确识别 EGFR 基因突变，提高临床获取肿瘤组织的能力。
本研究的重要发现：EG-P-LMB 可有效捕获肺癌患者外周血中的 CTC。进行了基因检测，并与组织进行了比较。解决了临床上肿瘤组织取样困难的问题。
