岩藻聚糖硫酸酯通过诱导 HSC-3 口腔鳞状癌细胞凋亡和自噬发挥抗肿瘤作用。
Antitumor Effects of Fucoidan Via Apoptotic and Autophagic Induction on HSC-3 Oral Squamous CellCarcinoma.
机构信息
Division of Anatomy, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand.
Division of Pharmacology, Department of Preclinical Science, Faculty of Medicine, Thammasat University, Pathumthani, 12120, Thailand.
出版信息
Asian Pac J Cancer Prev. 2020 Aug 1;21(8):2469-2477. doi: 10.31557/APJCP.2020.21.8.2469.
OBJECTIVE
Many studies suggested that fucoidan has anticancer potential. The objective of the present study was to determine the cytotoxic effects and mechanism of cell death induced by fucoidan extracted from Fucus vesiculosus on HSC-3 oral squamous cell carcinoma.
METHODS
HSC-3 cells were treated with 0, 100, 200, and 400 μg/mL of fucoidan. Cell viability was measured using MTT assay. Apoptosis and cell cycle were measured with a flow cytometry-based assay. Chromatin condensation and nuclear fragmentation were determined using Hoechst 33342 staining. Mitochondrial membrane potential (ΔΨm) was determined using the JC-1 kit. The apoptotic, anti-apoptotic, and autophagic markers study were done by western blot analysis.
RESULTS
the viable cell number of treated HSC-3 cells was decreased. Moreover, treated cells were arrested in the G0/G1 phase. Annexin V/PI staining revealed that fucoidan could induce apoptosis in HSC-3 cells. Western blot analysis suggested the up-regulation of apoptotic markers including cleaved caspase-3, cleaved PARP, Bax, and autophagic markers including LC3-II and Beclin-1 but down-regulation of anti-apoptotic markers, Bcl-2. Fucoidan could disturb ΔΨm and induce chromatin condensation with nuclear fragmentation.
CONCLUSION
fucoidan has potential in anticancer properties against HSC-3 cells manifested by the induction of apoptosis, cell cycle arrest, and autophagy.
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目的
许多研究表明褐藻糖胶具有抗癌潜力。本研究旨在确定从泡叶藻中提取的褐藻糖胶对口腔鳞状细胞癌细胞 HSC-3 的细胞毒性作用和细胞死亡机制。
方法：用 0、100、200 和 400μg/ml 的褐藻糖胶处理 HSC-3 细胞。用 MTT 法测定细胞活力。用流式细胞术检测细胞凋亡和细胞周期。用 Hoechst 33342 染色法检测染色质浓缩和核片段化。用 JC-1 试剂盒测定线粒体膜电位（ΔΨm）。通过 Western blot 分析研究凋亡、抗凋亡和自噬标志物。
结果：处理后的 HSC-3 细胞的活细胞数减少。此外，处理后的细胞被阻滞在 G0/G1 期。Annexin V/PI 染色显示褐藻糖胶可诱导 HSC-3 细胞凋亡。Western blot 分析表明，凋亡标志物包括 cleaved caspase-3、cleaved PARP、Bax 和自噬标志物包括 LC3-II 和 Beclin-1 的表达上调，但抗凋亡标志物 Bcl-2 的表达下调。褐藻糖胶可干扰ΔΨm 并诱导染色质浓缩和核片段化。
结论：褐藻糖胶对 HSC-3 细胞具有抗癌作用，表现为诱导细胞凋亡、细胞周期阻滞和自噬。
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