Haas G G, D'Cruz O J
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City.
Am J Reprod Immunol Microbiol. 1988 Jan;16(1):28-36. doi: 10.1111/j.1600-0897.1988.tb00173.x.
Human sperm, erythrocytes, and seminal plasma from the blood and semen of 20 men of known ABH, Lewis, and secretor phenotypes were assayed for ABH blood group antigens. A 1:1 correlation was found between the presence of ABH antigens in seminal plasma and on sperm and if the man had a secretor phenotype. Sperm from nonsecretors or from men of dissimilar ABO blood type could adsorb A antigen when incubated with A antigen-containing seminal plasma. The level of ABH antigens in seminal plasma correlated with the level of ABH antigens on the sperm surface. ABH antigens in semen were present only on a minority of spermatozoa as detected by flow cytometry, and the majority of these sperm were not in the swimup fraction. ABH antigens were not present on sperm within the seminiferous tubules of human testicular material. It was hypothesized that ABH antigens found on human sperm were adsorbed from seminal plasma on a minority of the sperm in an ejaculate.
对20名已知ABH、Lewis和分泌型表型男性的血液和精液中的人类精子、红细胞及精浆进行ABH血型抗原检测。结果发现，精浆和精子上ABH抗原的存在与男性是否具有分泌型表型之间存在1:1的相关性。非分泌型男性或ABO血型不同男性的精子与含A抗原的精浆孵育时可吸附A抗原。精浆中ABH抗原水平与精子表面ABH抗原水平相关。通过流式细胞术检测发现，精液中的ABH抗原仅存在于少数精子上，且这些精子中的大多数不在上浮部分。在人类睾丸组织的生精小管内的精子上未发现ABH抗原。据推测，人类精子上发现的ABH抗原是从射精中少数精子的精浆中吸附而来的。
