Departamento de Morfologia e Fisiologia Animal (DMFA), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, 52171-900, Recife, PE, Brazil; Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan St., Indianapolis, IN 46202, USA.
Departamento de Biologia (DB), Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros s/n, Dois Irmãos, 52171-900 Recife, PE, Brazil; Department of Biology, Indiana University Purdue University Indianapolis, 723 West Michigan St., Indianapolis, IN 46202, USA.
Neurotoxicol Teratol. 2020 Nov-Dec;82:106928. doi: 10.1016/j.ntt.2020.106928. Epub 2020 Aug 27.
Protective effects of quercetin (QUE), polydatin (POL), and folic acid (FA) and their mixtures were tested using zebrafish to model fetal alcohol spectrum disorder in this study. Zebrafish embryos were exposed to 150 mM ethanol for 6 or 22 h and co-treated with QUE, POL, FA, and their mixtures (37.5-100.0 μM). Epiboly progression, teratogenic effects, and behavior were evaluated. Ethanol exposure reduced epiboly, and FA and QUE protected against these ethanol-induced defects. POL did not reduce epiboly defects. The mixture QUE + FA showed a possible antagonistic effect. The observed teratogenic effects were similar in all ethanol exposed groups. QUE, FA and QUE + POL reduced the percentage of affected animals, but treatments did not eliminate teratogenic effects. Behavioral measurements were divided into small (between 4 and 8 mm/s) and high swimming activity (>8 mm/s). All experimental groups displayed a reduction in small swimming activity as compared to control and ethanol groups when exposed to bright light. Additionally, larvae exposed to ethanol were more inhibited than control, not showing a habituation period (after 60 min of experiment) in high swimming activity. Chemical treatments like QUE and POL reduced behavioral defects induced by ethanol exposure. In conclusion, this study presents new evidence that QUE, POL, FA and their mixtures partially protected epiboly, teratogenic, and behavioral defects induced by ethanol exposure. QUE, FA and QUE + POL were more effective in reducing these defects than the other studied compounds and mixtures.
本研究采用斑马鱼模型来模拟胎儿酒精谱系障碍，测试了槲皮素（QUE）、虎杖苷（POL）和叶酸（FA）及其混合物的保护作用。将斑马鱼胚胎暴露于 150 mM 乙醇中 6 或 22 小时，并与 QUE、POL、FA 及其混合物（37.5-100.0 μM）共同处理。评估了胚环运动、致畸作用和行为。乙醇暴露会降低胚环运动，而 FA 和 QUE 可防止这些乙醇引起的缺陷。POL 不会减少胚环运动缺陷。QUE+FA 的混合物显示出可能的拮抗作用。观察到的致畸作用在所有暴露于乙醇的组中相似。QUE、FA 和 QUE+POL 降低了受影响动物的百分比，但治疗并未消除致畸作用。行为测量分为小（4-8 mm/s）和高游泳活动（>8 mm/s）。与对照和乙醇组相比，所有实验组在暴露于强光下时，小游泳活动均减少。此外，与对照相比，暴露于乙醇的幼虫更受抑制，在高游泳活动中没有表现出习惯期（实验 60 分钟后）。像 QUE 和 POL 这样的化学处理可以减少乙醇暴露引起的行为缺陷。总之，本研究提供了新的证据，表明 QUE、POL、FA 及其混合物部分保护了乙醇暴露引起的胚环运动、致畸和行为缺陷。与其他研究的化合物和混合物相比，QUE、FA 和 QUE+POL 更有效地减少了这些缺陷。
