Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Academy of Fishery Sciences, Nanning 530021, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China.
Gene. 2021 Jan 5;764:145090. doi: 10.1016/j.gene.2020.145090. Epub 2020 Aug 27.
Litopenaeus vannamei (L. vannamei) is one of the most widely cultured shrimp species in the world. The species often suffers from cold stress. To understand the molecular mechanism of cold tolerance, we performed transcriptomic analysis on two contrasting cultivars of L. vannamei, namely, cold-tolerant Guihai 2 (GH2) and cold-sensitive Guihai1 (GH1), under a control temperature (28 °C), cold stress (16 °C), and recovery to 28 °C. A total of 84.5 Gb of sequences were generated from 12 L. vannamei hepatopancreas libraries. The de-novo assembly generated a total of 143,029 unigenes with a mean size of 1,052 bp and an N50 of 2,604 bp, of which 34.08% were annotated in the Nr database. We analyzed the differentially expressed genes (DEGs) between nine comparison groups and detected a total of 21,026 DEGs. KEGG pathways, including lysosome, sphingolipid metabolism and nitrogen metabolism, were significantly enriched by DEGs between different temperatures in GH2. Furthermore, eight of the most significantly DEGs under cold stress from the transcriptomic analysis were selected for quantitative real-time PCR (qPCR) validation. Overall, we compared gene expression changes under cold stress in cold-tolerant and cold-sensitive L. vannamei for the first time. The results may further extend our understanding of the cold stress-response mechanism in L. vannamei.
凡纳滨对虾（L. vannamei）是世界上养殖最广泛的虾类之一。该物种常遭受冷应激。为了了解耐寒性的分子机制，我们对两个不同的凡纳滨对虾品种（耐冷的桂海 2 号（GH2）和敏感的桂海 1 号（GH1））进行了转录组分析，分别在对照温度（28°C）、冷应激（16°C）和恢复到 28°C 下。从 12 个凡纳滨对虾肝胰腺文库中总共生成了 84.5Gb 的序列。从头组装总共生成了 143029 个 unigenes，平均大小为 1052bp，N50 为 2604bp，其中 34.08%在 Nr 数据库中被注释。我们分析了 9 个比较组之间的差异表达基因（DEGs），共检测到 21026 个 DEGs。KEGG 通路，包括溶酶体、鞘脂代谢和氮代谢，在 GH2 中不同温度之间的差异基因中显著富集。此外，从转录组分析中选择了 8 个在冷应激下表达最显著的 DEGs 进行定量实时 PCR（qPCR）验证。总的来说，我们首次比较了耐寒和敏感凡纳滨对虾在冷应激下的基因表达变化。这些结果可能进一步扩展我们对凡纳滨对虾冷应激反应机制的理解。
