El-Sayed Shimaa Abd El-Salam, AbouLaila Mahmoud, ElKhatam Ahmed, Abdel-Wahab Azaa, Rizk Mohamed Abdo
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt.
Department of Parasitology, Faculty of Veterinary Medicine, Damanhour University, Damanhour 22511, El Behera, Egypt.
Vet Parasitol Reg Stud Reports. 2020 Jul;21:100449. doi: 10.1016/j.vprsr.2020.100449. Epub 2020 Aug 13.
In the present study, we conducted an epidemiological survey of Theileria equi, with sequencing analysis of the PCR product using blood-DNA samples collected from donkeys (n = 149) reared in different Egyptian provinces in Lower Egypt (Menoufia and Mersa Matruh) and middle Egypt (Giza). All animals were tested for the presence of T. equi parasite using species-specific PCR assay targeting the Equi merozoite antigen-1 (EMA-1). Nine- (6.04%) samples were positive for T. equi. The highest positive rate for infection was detected in Giza zoological garden (10.16%). Egyptian EMA-1 gene sequence exhibited a high identity with gene sequence from Italy, Japan, South Africa, Indian and Israel, the Palestinian Authority. In conclusion, data presented here revealed for the presence of T. equi in donkeys in two provinces of Egypt either in form of acute infection or carriers. These findings have economic significance and indicate the importance of introducing effective prevention and control strategies throughout Egypt to minimize the prevalence of equine piroplasmosis caused by T. equi.
在本研究中，我们对马泰勒虫进行了流行病学调查，使用从埃及下埃及（米努夫省和马特鲁港）和中埃及（吉萨省）不同省份饲养的驴（n = 149）采集的血液DNA样本对PCR产物进行测序分析。使用针对马裂殖子抗原-1（EMA-1）的种特异性PCR检测法对所有动物进行马泰勒虫寄生虫检测。9份（6.04%）样本马泰勒虫呈阳性。在吉萨动物园检测到最高感染阳性率（10.16%）。埃及EMA-1基因序列与来自意大利、日本、南非、印度、以色列、巴勒斯坦权力机构的基因序列具有高度同一性。总之，此处呈现的数据显示埃及两个省份的驴中存在马泰勒虫，其形式为急性感染或携带者。这些发现具有经济意义，并表明在埃及各地引入有效的预防和控制策略以尽量减少由马泰勒虫引起的马焦虫病流行的重要性。
