Song Fucun, Yang Yang, Liu Jixiang
Department of Otolaryngology Head and Neck Surgery, Tianjin Union Medical Center, Tianjin 300121, P.R. China.
Oncol Lett. 2020 Oct;20(4):69. doi: 10.3892/ol.2020.11930. Epub 2020 Jul 29.
Laryngeal cancer is a common head and neck cancer that effects the quality of life of those affected. Early diagnosis and treatment are vital to minimize the harmful effects of laryngeal cancer, which can improve the survival rate of patients following surgery and retain the voice function of the larynx. The purpose of the present study was to explore the molecular mechanism of the development of laryngeal cancer and to determine the biomarker for the diagnosis and treatment of laryngeal cancer. Reverse transcription-quantitative PCR (RT-qPCR) and The Cancer Genome Atlas database analysis were used to confirm high expression of TMEM158 in laryngeal cancer. The effects of TMEM158 and miR-548ac was investigated through and assays (MTT assay, colony-formation assay, flow cytometry assay, western blotting and tumor xenograft assay). Luciferase reporter assay, western blotting and RTq-PCR were used to confirm that miR-548 directly targeted the 3'-untranslated region of TMEM158 and inhibited TMEM158 expression. Taken together, the present results suggest that miR-548ac functions as a crucial cancer suppressor in laryngeal cancer, which induces apoptosis in laryngeal cancer cells by suppressing TMEM158. Thus, miR-548ac may be a potential target for the treatment of laryngeal cancer.
喉癌是一种常见的头颈癌，会影响患者的生活质量。早期诊断和治疗对于将喉癌的有害影响降至最低至关重要，这可以提高患者术后的生存率并保留喉部的发声功能。本研究的目的是探索喉癌发生发展的分子机制，并确定用于喉癌诊断和治疗的生物标志物。采用逆转录定量PCR（RT-qPCR）和癌症基因组图谱数据库分析来证实TMEM158在喉癌中高表达。通过MTT法、集落形成试验、流式细胞术检测、蛋白质印迹法和肿瘤异种移植试验研究了TMEM158和miR-548ac的作用。荧光素酶报告基因检测、蛋白质印迹法和RT-qPCR用于证实miR-548直接靶向TMEM158的3'-非翻译区并抑制TMEM158的表达。综上所述，目前的结果表明miR-548ac在喉癌中作为一种关键的抑癌基因发挥作用，它通过抑制TMEM158诱导喉癌细胞凋亡。因此，miR-548ac可能是喉癌治疗的一个潜在靶点。
