Deng Yuanle, Zhang Qianyu, Li Yali, Wang Liqun, Yang Shuping, Chen Xiaotong, Gan Cailin, He Fang, Ye Tinghong, Yin Wenya
Department of Nutrition, Food Safety and Toxicology, West China School of Public Health and Healthy Food Evaluation Research Center, Sichuan University, Chengdu, Sichuan 610064, P.R China.
State Key of Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Chengdu, Sichuan 610064, P.R China.
Oncol Lett. 2020 Oct;20(4):116. doi: 10.3892/ol.2020.11977. Epub 2020 Aug 12.
Pectolinarigenin a plant secondary metabolite that has various biological effects, including the inhibition of melanogenesis and tumor growth. Melanoma has a high degree of malignancy, with rapid metastasis and severe drug resistance, explaining the need for new candidate drugs that inhibit tumor growth and metastasis. However, the pharmacological action and mechanism of pectolinarigenin on the growth and metastasis of melanoma remain elusive. Thus, the present study aimed to investigate the role of pectolinarigenin in melanoma cell proliferation, apoptosis, migration and invasion. Apoptotic and metastasis-associated proteins were analyzed using western blotting. The results demonstrated that pectolinarigenin treatment resulted in growth inhibition and apoptosis induction in melanoma cells, arising from the loss of mitochondrial transmembrane potential, reactive oxygen species and the altered expression of apoptosis-associated proteins. In addition, wound-healing and Transwell assays demonstrated the potential of pectolinarigenin to impair the migration and invasion of melanoma cells in accordance with the changes in the expression of the associated proteins. Therefore, the results of the present study suggested that pectolinarigenin may serve a pivotal role in promoting melanoma cell apoptosis and reducing metastasis, and may thus be a promising potential candidate for an anti-melanoma treatment strategy.
橙皮素是一种植物次生代谢产物，具有多种生物学效应，包括抑制黑色素生成和肿瘤生长。黑色素瘤具有高度恶性，转移迅速且耐药性严重，这解释了需要新的抑制肿瘤生长和转移的候选药物。然而，橙皮素对黑色素瘤生长和转移的药理作用及机制仍不清楚。因此，本研究旨在探讨橙皮素在黑色素瘤细胞增殖、凋亡、迁移和侵袭中的作用。使用蛋白质免疫印迹法分析凋亡和转移相关蛋白。结果表明，橙皮素处理导致黑色素瘤细胞生长抑制和凋亡诱导，这是由于线粒体跨膜电位丧失、活性氧以及凋亡相关蛋白表达改变所致。此外，伤口愈合和Transwell实验表明，橙皮素具有根据相关蛋白表达变化损害黑色素瘤细胞迁移和侵袭的潜力。因此，本研究结果表明，橙皮素可能在促进黑色素瘤细胞凋亡和减少转移中起关键作用，因此可能是抗黑色素瘤治疗策略的有希望的潜在候选药物。
