Department of Biotechnology, Sri Sankara Arts and Science College, Kanchipuram, India.
Department of Physics, Sri Sankara Arts and Science College, Kanchipuram, India.
Bioelectromagnetics. 2020 Oct;41(7):526-539. doi: 10.1002/bem.22292. Epub 2020 Aug 31.
The ability of extremely low, time-varying electromagnetic field (EMF) to improve germination efficacy was studied in Foxtail millet (Setaria italica) seeds using response surface methodology. An optimal factorial central composite design was chosen to optimize the EMF with three critical factors, viz. frequency, intensity, and duration. The adequacy of the model and fitness was evaluated by analysis of variance and regression coefficients. This model suggested that the factors, frequency, and intensity had a significant impact on germination. Optimal conditions for germination were observed to be 10 Hz frequency, 30,007 nT intensity, and 30-min duration with an observed germination percentage of 93.0, and a predicted germination percentage of 92.92. Magneto-priming was found to increase the germination efficacy (15.66%), shoot length (27.78%), total seedling length (20.30%), seedling dry mass (26.49%), and water uptake (34.48% at 80 min) showing significant output when compared with the control and positive controls. Remarkable improvements were observed in germination parameters such as vigor index-1 (39.14%), vigor index-2 (46.28%), speed of germination (27.52%), and emergence index (12.50%). Magneto-priming was found to reduce the levels of germination-specific enzymes, viz. α-amylase, protease, and dehydrogenase, while it enhanced the levels of antioxidant enzymes, viz. catalase (114.63%) and superoxide dismutase (19.62%), triggering fast germination and early vigor of seedlings. This study clearly showed that EMF priming significantly improved the germination effect and other characteristics of Foxtail millet seeds. Bioelectromagnetics. © 2020 Bioelectromagnetics Society.
采用响应面法研究了极低频、时变电磁场（EMF）对黍（Setaria italica）种子发芽效果的影响。选择最佳的析因中心组合设计来优化 EMF，其中三个关键因素是频率、强度和时间。通过方差分析和回归系数评估模型的充分性和拟合度。该模型表明，频率和强度这两个因素对发芽有显著影响。观察到最佳发芽条件为 10 Hz 频率、30007 nT 强度和 30 分钟时间，发芽率为 93.0%，预测发芽率为 92.92%。磁引发被发现可提高发芽效果（15.66%）、芽长（27.78%）、总苗长（20.30%）、苗干重（26.49%）和吸水（80 分钟时 34.48%），与对照和阳性对照相比，表现出显著的效果。发芽参数如活力指数-1（39.14%）、活力指数-2（46.28%）、发芽速度（27.52%）和发芽指数（12.50%）也有显著提高。磁引发降低了发芽特异性酶如α-淀粉酶、蛋白酶和脱氢酶的水平，同时提高了抗氧化酶如过氧化氢酶（114.63%）和超氧化物歧化酶（19.62%）的水平，从而促进了种子的快速发芽和幼苗的早期活力。这项研究清楚地表明，EMF 引发显著提高了黍种子的发芽效果和其他特性。生物电磁学。2020 年生物电磁学学会。
