Grigsby Anthony, Kelly Brendan J, Sanscrainte Neil D, Becnel James J, Short Sarah M
Department of Entomology, The Ohio State University.
USDA/ARS Center for Medical, Agricultural, and Veterinary Entomology.
J Vis Exp. 2020 Aug 13(162). doi: 10.3791/61574.
Edhazardia aedis is a microsporidian parasite of Aedes aegypti mosquitoes, a disease vector that transmits multiple arboviruses which cause millions of disease cases each year. E. aedis causes mortality and reduced reproductive fitness in the mosquito vector and has been explored for its potential as a biocontrol agent. The protocol we present for culturing E. aedis is based on its natural infection cycle, which involves both horizontal and vertical transmission at different life stages of the mosquito host. Ae. aegypti mosquitoes are exposed to spores in the larval stage. These infected larvae then mature into adults and transmit the parasite vertically to their offspring. Infected offspring are then used as a source of spores for future horizontal transmission. Culturing E. aedis can be challenging to the uninitiated given the complexities of the parasite's life cycle, and this protocol provides detailed guidance and visual aids for clarification.
伊蚊埃哈兹孢虫是埃及伊蚊的一种微孢子虫寄生虫，埃及伊蚊是一种病媒，可传播多种虫媒病毒，每年导致数百万病例。伊蚊埃哈兹孢虫会导致蚊媒死亡并降低其生殖适应性，人们已对其作为生物防治剂的潜力进行了探索。我们提出的培养伊蚊埃哈兹孢虫的方案基于其天然感染周期，该周期涉及蚊宿主不同生命阶段的水平传播和垂直传播。埃及伊蚊幼虫接触孢子。这些受感染的幼虫随后发育成成虫，并将寄生虫垂直传播给它们的后代。受感染的后代随后被用作孢子来源，用于未来的水平传播。鉴于寄生虫生命周期的复杂性，对于新手来说，培养伊蚊埃哈兹孢虫可能具有挑战性，本方案提供了详细的指导和直观教具以作说明。
