双光子光遗传学刺激果蝇神经元。

Two-Photon Optogenetic Stimulation of Drosophila Neurons.

机构信息

Wolfson Institute for Biomedical Research and Department of Neuroscience, Physiology, and Pharmacology, University College London, London, UK.

Department of Neuroscience, Yale School of Medicine, New Haven, CT, USA.

出版信息

Methods Mol Biol. 2021;2191:97-108. doi: 10.1007/978-1-0716-0830-2_7.

DOI:10.1007/978-1-0716-0830-2_7

PMID:32865741

Abstract

Optogenetics enables experimental control over neural activity using light. Channelrhodopsin and its variants are typically activated using visible light excitation but can also be activated using infrared two-photon excitation. Two-photon excitation can improve the spatial precision of stimulation in scattering tissue but has several practical limitations that need to be considered before use. Here we describe the methodology and best practices for using two-photon optogenetic stimulation of neurons within the brain of the fruit fly, Drosophila melanogaster, with an emphasis on projection neurons of the antennal lobe.

摘要

光遗传学使用光来实现对神经活动的实验控制。通道视紫红质及其变体通常使用可见光激发来激活，但也可以使用红外双光子激发来激活。双光子激发可以提高散射组织中刺激的空间精度，但在使用前需要考虑几个实际限制。本文描述了在果蝇（Drosophila melanogaster）大脑中使用双光子光遗传学刺激神经元的方法和最佳实践，重点介绍了触角叶中的投射神经元。