使用通道蛋白视紫红质对中枢杏仁核进行光遗传学刺激。
Optogenetic Stimulation of the Central Amygdala Using Channelrhodopsin.
机构信息
Neuroscience & Behavior Program, Wesleyan University, Middletown, CT, USA.
Department of Psychology, Wesleyan University, Middletown, CT, USA.
出版信息
Methods Mol Biol. 2021;2191:351-376. doi: 10.1007/978-1-0716-0830-2_20.
Optogenetics allows for the targeted temporary inhibition or stimulation of specific brain regions in vivo with precise temporal resolution. Here, we describe the steps to perform intracranial optogenetic surgery in rodents as well as instructions to build an optogenetic headcap and set up an optogenetic testing environment to conduct experiments. Behavioral studies have implemented these methods to stimulate the central amygdala (CeA) to create an addictive-like preference for reward.
光遗传学允许在体内以精确的时间分辨率靶向性地暂时抑制或刺激特定的脑区。在这里，我们描述了在啮齿动物中进行颅内光遗传学手术的步骤，以及构建光遗传学头套和设置光遗传学测试环境以进行实验的说明。行为研究已经实施了这些方法来刺激中央杏仁核（CeA），以产生对奖励的类似成瘾的偏好。
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