Laboratoire de Pharmacologie et Physiopathologie Expérimentale, UMR Qualisud, CIRAD-Université de Montpellier, Montpellier, France; Laboratoire de Botanique, Phytochimie et Mycologie, CEFE, CNRS-Université de Montpellier-Université Paul-Valéry Montpellier-EPHE-IRD, Montpellier, France; Laboratoire de Technologie Alimentaire du Département de Génie Chimique de l'Université Gamal Abdel Nasser de Conakry, BP 1147, Conakry, Guinée.
Laboratoire d'Immunologie, UMR Qualisud, CIRAD-Université de Montpellier, Montpellier, France.
Int J Med Mushrooms. 2020;22(6):535-546. doi: 10.1615/IntJMedMushrooms.2020034864.
Lentinus edodes (= Lentinula edodes) is an edible mushroom grown and marketed for centuries due to its nutritional and medicinal properties. L. edodes has multiple pharmacological activities as an antioxidant and anti-inflammatory. Few studies were performed taking into account the influence of culture conditions to optimize the biological properties of L. edodes on human health. Our work focused on the comparison of antioxidant capacity and anti-inflammatory activity of L. edodes fruit bodies cultivated by three mushroom producers in the French Occitanie region using the same strain in various growing conditions (organic and nonorganic). Sequential extraction was performed on freeze-dried fungal materials. All extracts have a quantifiable but moderate antioxidant activity measured via DPPH and ORAC tests. The anti-inflammatory activity of the ethanol and aqueous extracts was evaluated on a model of inflammatory macrophages. The ethanol extracts inhibit NO production in a dose-dependent manner when the cells are pretreated for 4 h with a 24 h stimulation time.
香菇（Lentinula edodes）是一种可食用的蘑菇，由于其营养价值和药用特性，已经被种植和销售了几个世纪。香菇具有抗氧化和抗炎等多种药理活性。考虑到培养条件的影响，以优化香菇对人类健康的生物特性的研究较少。我们的工作重点是比较三种法国奥克西塔尼地区的蘑菇生产商在不同的培养条件（有机和非有机）下使用相同菌株栽培的香菇子实体的抗氧化能力和抗炎活性。对冻干真菌材料进行了顺序提取。所有提取物均具有可量化但适中的抗氧化活性，可通过 DPPH 和 ORAC 测试进行测量。用炎症巨噬细胞模型评估了乙醇和水提物的抗炎活性。当细胞用 24 小时刺激时间预处理 4 小时时，乙醇提取物以剂量依赖性方式抑制 NO 的产生。
