State Key Laboratory of Crop Stress Biology for Arid Areas/Shaanxi Key Laboratory of Apple, College of Horticulture, Northwest A&F University, Yangling 712100, China.
Int J Mol Sci. 2020 Aug 27;21(17):6182. doi: 10.3390/ijms21176182.
Ethylene plays an important role in stress adaptation and fruit ripening. Acireductone dioxygenase (ARD) is pivotal for ethylene biosynthesis. However, the response of ARD to fruit ripening or cold stress is still unclear. In this study, we identified three members of ARD family, and expression profile analysis revealed that the transcript level of was induced during apple fruit ripening and after apple plants were being treated with cold stress. To investigate its function in cold tolerance and fruit ripening, was ectopically expressed in cultivar 'Micro-Tom', which has been considered as an excellent model plant for the study of fruit ripening. At the cellular level, the MdARD protein expressed throughout epidermal cells. Overexpression of in tomato demonstrated that regulates the ethylene and carotenoid signaling pathway, increases ethylene and carotenoid concentrations, and accelerates fruit ripening. Furthermore, increased the antioxidative ability and cold hardiness in tomato. To conclude, may potentially be used in apple breeding to accelerate fruit ripening and increase cold hardiness.
乙烯在应激适应和果实成熟中起着重要作用。乙烯生物合成的关键酶是 ACC 氧化酶（ACO）。然而，ACO 对果实成熟或冷胁迫的响应仍不清楚。本研究鉴定了 ACO 家族的三个成员，表达谱分析表明，在苹果果实成熟过程中和苹果植株受到冷胁迫后，的转录水平被诱导。为了研究其在耐冷性和果实成熟中的功能，在“Micro-Tom”品种中异源表达，该品种被认为是研究果实成熟的优秀模式植物。在细胞水平上，MdARD 蛋白在表皮细胞中表达。在番茄中过表达表明，通过调节乙烯和类胡萝卜素信号通路，增加乙烯和类胡萝卜素的浓度，加速果实成熟。此外，提高了番茄的抗氧化能力和抗寒性。总之，可能在苹果育种中用于加速果实成熟和提高抗寒性。
