应用 Tc-DMSA 评价梗阻性输尿管模型中的肾功能。
Evaluation of Renal Function in Obstructed Ureter Model Using Tc-DMSA.
机构信息
Onnuri Animal Medical Center, Cheongju, Republic of Korea.
College of Veterinary Medicine, Chungbuk National University, Cheongju, Republic of Korea.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2431-2435. doi: 10.21873/invivo.12057.
BACKGROUND/AIM: Urinary obstruction is a condition of impaired urinary drainage, which may result in progressive renal deterioration. This study applied Tc-labeled dimercaptosuccinic acid (Tc-DMSA) renal scintigraphy to a rabbit model of right ureter obstruction and evaluated its utility in studying obstructive renal diseases.
MATERIALS AND METHODS
Complete unilateral ureter obstruction in rabbits was generated by complete ligation of the right ureter. Renal function was investigated during a 4-week post-obstruction period by obtaining planar images of Tc-DMSA activity following ear vein injection. Renal blood perfusion was evaluated by non-invasive scintigraphy in conjunction with parallel histological and hematological examinations.
RESULTS
Renal perfusion was remarkably and rapidly reduced in the ureter-obstructed kidneys. During the experimental period, the size of left kidney appeared normal in the scintigraphic images, but the ureter-obstructed right kidney progressively became larger. Histopathological examination showed flattening and atrophy of tubules, enlargement of interstitial areas, accumulation of extracellular martices and infiltration of inflammatory cells in the obstreucted kidney.
CONCLUSION
Tc-DMSA scintigraphy is a sensitive, non-invasive method to assess renal function in unilateral kidney diseases.
背景/目的：尿路梗阻是一种尿路引流受损的情况，可能导致进行性肾功能恶化。本研究应用 Tc 标记的二巯丁二酸（Tc-DMSA）肾闪烁显像术对单侧输尿管梗阻的兔模型进行研究，并评估其在阻塞性肾病研究中的应用价值。
材料和方法
通过完全结扎右侧输尿管，在兔中产生完全性单侧输尿管梗阻。通过耳静脉注射 Tc-DMSA 活性后获得平面图像，在梗阻后 4 周期间研究肾功能。通过非侵入性闪烁显像术结合平行的组织学和血液学检查评估肾血流灌注。
结果
梗阻侧肾脏的肾血流明显且迅速减少。在实验期间，闪烁显像图像中左侧肾脏的大小正常，但右侧梗阻的肾脏逐渐增大。组织病理学检查显示梗阻侧肾脏的肾小管扁平萎缩，间质区扩大，细胞外基质积聚，炎症细胞浸润。
结论
Tc-DMSA 闪烁显像术是评估单侧肾脏疾病肾功能的一种敏感、非侵入性方法。
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