The Rogel Cancer Center, University of Michigan, Ann Arbor, MI 48109, USA.
Department of Epidemiology, School of Public Health, University of Michigan, Ann Arbor, MI 48109, USA.
Nutrients. 2020 Aug 29;12(9):2638. doi: 10.3390/nu12092638.
Blacks experience disproportionate head and neck cancer (HNC) recurrence and mortality compared to Whites. Overall, vitamin D status is inversely associated to HNC pointing to a potential protective linkage. Although hypovitaminosis D in Blacks is well documented it has not been investigated in Black HNC patients. Thus, we conducted a prospective pilot study accessing vitamin D status in newly diagnosed HNC patients stratified by race and conducted in vitro studies to investigate mechanisms associated with potential cancer inhibitory effects of vitamin D. Outcome measures included circulating levels of vitamin D, related nutrients, and risk factor characterization as well as dietary and supplemental estimates. Vitamin D-based in vitro assays utilized proteome and microRNA (miR) profiling. Nineteen patients were enrolled, mean circulating vitamin D levels were significantly reduced in Black compared to White HNC patients, 27.3 and 20.0 ng/mL, respectively. Whites also supplemented vitamin D more frequently than Blacks who had non-significantly higher vitamin D from dietary sources. Vitamin D treatment of HNC cell lines revealed five significantly altered miRs regulating genes targeting multiple pathways in cancer based on enrichment analysis (i.e., negative regulation of cell proliferation, angiogenesis, chemokine, MAPK, and WNT signaling). Vitamin D further altered proteins involved in cancer progression, metastasis and survival supporting a potential role for vitamin D in targeted cancer prevention.
与白人相比，黑人经历了不成比例的头颈部癌症（HNC）复发和死亡率。总体而言，维生素 D 状态与 HNC 呈负相关，表明存在潜在的保护联系。尽管黑人维生素 D 缺乏症已有充分记录，但尚未在黑人 HNC 患者中进行研究。因此，我们进行了一项前瞻性试点研究，对按种族分层的新诊断的 HNC 患者的维生素 D 状态进行了评估，并进行了体外研究，以调查与维生素 D 潜在抗癌作用相关的机制。结果测量包括循环维生素 D 水平、相关营养素和危险因素特征以及饮食和补充剂估计。基于维生素 D 的体外测定采用蛋白质组和 microRNA (miR) 分析。共纳入 19 名患者，黑人 HNC 患者的循环维生素 D 水平明显低于白人患者，分别为 27.3 和 20.0 ng/mL。白人补充维生素 D 的频率也高于黑人，而黑人从饮食中获得的维生素 D 则较高，但无统计学意义。维生素 D 治疗 HNC 细胞系显示，根据富集分析，有五个明显改变的 miRs 调节了针对癌症中多个途径的基因（即细胞增殖、血管生成、趋化因子、MAPK 和 WNT 信号的负调控）。维生素 D 进一步改变了与癌症进展、转移和生存相关的蛋白质，支持维生素 D 在靶向癌症预防中的潜在作用。
