Dessi S, Chiodino C, Batetta B, Armeni M, Mulas M F, Pani P
Istituto di Farmacologia e Patologia Biochimica, University of Cagliari, Italy.
Pathology. 1988 Jan;20(1):53-7. doi: 10.3109/00313028809085197.
DNA synthesis, cholesterogenesis and the enzymes of the hexosemonophosphate (HMP) shunt pathway were investigated in liver of diabetic rats treated with insulin and in fasted/re-fed rats. Both insulin and refeeding were found to induce liver cell proliferation, accompanied by a remarkable increase in cholesterogenesis. An enhancement of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (G6PD) activities was also found in insulin-treated diabetic rats and in re-fed rats, supporting the concept that these two enzymes are involved in the proliferative process. Since insulin did not exert the same biochemical effects in a non replicating cell population, such as in insulin-treated normal rats, these studies provide new evidence of a close correlation between DNA, cholesterol synthesis and HMP shunt enzymes during cell proliferation.
在接受胰岛素治疗的糖尿病大鼠肝脏以及禁食/再喂养大鼠中，对DNA合成、胆固醇生成及磷酸己糖（HMP）支路途径的酶进行了研究。发现胰岛素和再喂养均能诱导肝细胞增殖，同时胆固醇生成显著增加。在接受胰岛素治疗的糖尿病大鼠和再喂养大鼠中，还发现葡萄糖-6-磷酸脱氢酶（G6PD）和6-磷酸葡萄糖酸脱氢酶（6PGD）活性增强，这支持了这两种酶参与增殖过程的观点。由于胰岛素在非复制细胞群体（如接受胰岛素治疗的正常大鼠）中未产生相同的生化效应，这些研究为细胞增殖过程中DNA、胆固醇合成及HMP支路酶之间密切相关提供了新证据。
