Guo Y S, Singh P, DeBouno J F, Thompson J C
Department of Surgery, University of Texas Medical Branch, Galveston 77550.
Pancreas. 1988;3(2):128-34. doi: 10.1097/00006676-198804000-00003.
Peptide YY (PYY) is a hormone released from gut after a meal. The objective of this study was to determine the effect of PYY on insulin release stimulated by either 2-deoxyglucose (2-DG) or neuropeptides in conscious dogs with gastric and duodenal fistulas. In control experiments dogs received either 2-DG (75 mg/kg i.v. bolus) or atropine (25 micrograms/kg bolus followed by 20 micrograms/kg/h i.v.) plus 2-DG (75 mg/kg i.v.) or bethanechol (80 micrograms/kg/h i.v.) or vasoactive intestinal peptide (VIP, 4 micrograms/kg i.v. bolus) or gastrin-releasing peptide (GRP, 400 pmol/kg/h i.v.) or tetragastrin (G4, 100 micrograms/dog, i.v. bolus). On separate days, PYY was also infused intravenously in combination with one of the above stimulants. Given intravenously, PYY (200, 400 pmol/kg/h) significantly inhibited 2-DG stimulated-insulin secretion in a dose-dependent fashion. This inhibitory effect also existed in the presence of atropine. Peptide YY (400 pmol/kg/h) depressed the insulin levels in response to GRP or G4 but failed to inhibit bethanechol- and VIP-stimulated insulin release. After administration of the above stimulants, PYY did not modify the blood sugar concentrations. These results demonstrated that PYY might inhibit the cephalic phase of insulin release from dogs triggered by 2-DG and by the neuropeptides GRP and G4. Thus, PYY may play a negative feedback regulatory role on insulin release.
肽YY(PYY)是进食后从肠道释放的一种激素。本研究的目的是确定PYY对有意识的患有胃和十二指肠瘘管的犬由2-脱氧葡萄糖(2-DG)或神经肽刺激的胰岛素释放的影响。在对照实验中,犬接受2-DG(静脉推注75mg/kg)或阿托品(静脉推注25μg/kg,随后以20μg/kg/h静脉输注)加2-DG(静脉推注75mg/kg)或氨甲酰甲胆碱(静脉输注80μg/kg/h)或血管活性肠肽(VIP,静脉推注4μg/kg)或胃泌素释放肽(GRP,静脉输注400pmol/kg/h)或四肽胃泌素(G4,100μg/犬,静脉推注)。在不同的日子里,PYY也与上述一种刺激剂联合静脉输注。静脉给予PYY(200、400pmol/kg/h)以剂量依赖的方式显著抑制2-DG刺激的胰岛素分泌。在阿托品存在的情况下这种抑制作用也存在。肽YY(400pmol/kg/h)降低了对GRP或G4反应的胰岛素水平,但未能抑制氨甲酰甲胆碱和VIP刺激的胰岛素释放。给予上述刺激剂后,PYY未改变血糖浓度。这些结果表明,PYY可能抑制犬由2-DG以及神经肽GRP和G4触发的胰岛素释放的头期。因此,PYY可能对胰岛素释放起负反馈调节作用。