Department of Obstetrics and Gynecology, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, No.1 Shuai Fu Road, Dongcheng District, Beijing, People's Republic of China.
Department of Gynecology, The First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing, China.
Cell Tissue Res. 2020 Dec;382(3):519-527. doi: 10.1007/s00441-020-03234-5. Epub 2020 Sep 2.
In order to evaluate the effects of human umbilical cord-derived stem cells (HUMSCs) on the biocompatibility of and tissue response to a polypropylene (PP) mesh (Gynemesh™ PS) implanted in rat vaginas, HUMSCs were isolated and characterized in vitro and then combined with Gynemesh™ PS to create a tissue-engineered mesh. This tissue-engineered mesh and pure PP mesh were implanted in the submucosae of the posterior vaginal walls of rats. Mesh/tissue complexes were harvested at 1, 4 and 12 weeks after implantation. Histological evaluations including an assessment of the inflammatory reaction, neovascularization and fibrosis around the mesh fibers were performed and real-time quantitative polymerase chain reaction (RT-PCR) was used to analyze the mRNA expression of genes involved in wound healing at the tissue-mesh interface. After being seeded onto the PP mesh scaffold, HUMSCs grew and proliferated well in vitro culture. One week after implantation, the HUMSC-seeded mesh elicited a greater inflammatory response than the pure PP mesh (3.33 ± 0.21 vs. 2.63 ± 0.18, p = 0.026), while 4 and 12 weeks after implantation, the inflammatory response in the HUMSC-seeded mesh was lower than that in the unseeded mesh (p < 0.05). At 12 weeks, the HUMSC-seeded mesh induced a lower expression of matrix metalloproteinase (MMP)-1 and a higher expression of anti-inflammatory cytokine interleukin (IL)-4. HUMSCs may decrease the inflammatory response and improve the biocompatibility of a conventional synthetic mesh and may have the potential to reduce postoperative complications such as mesh exposure or erosion.
为了评估人脐带间充质干细胞（HUMSCs）对植入大鼠阴道的聚丙烯（PP）网（Gynemesh™ PS）的生物相容性和组织反应的影响，HUMSCs 在体外被分离和鉴定，然后与 Gynemesh™ PS 结合形成组织工程网。该组织工程网和纯 PP 网被植入大鼠阴道后壁的黏膜下层。在植入后 1、4 和 12 周收获网/组织复合物。进行组织学评估，包括对网纤维周围的炎症反应、新生血管形成和纤维化的评估，并使用实时定量聚合酶链反应（RT-PCR）分析组织-网界面处参与伤口愈合的基因的 mRNA 表达。HUMSCs 在体外培养中生长和增殖良好。植入后 1 周，HUMSC 接种的网比纯 PP 网引起更大的炎症反应（3.33±0.21 对 2.63±0.18，p=0.026），而植入后 4 和 12 周，HUMSC 接种的网的炎症反应低于未接种的网（p<0.05）。在 12 周时，HUMSC 接种的网诱导基质金属蛋白酶（MMP）-1 的表达降低，抗炎细胞因子白细胞介素（IL）-4 的表达升高。HUMSCs 可能会降低炎症反应，提高传统合成网的生物相容性，并有可能减少术后并发症，如网暴露或侵蚀。
