Zhang Shaolun, Shen Manman, Yin Haotong, Huang Haoling, Li Tao, Zhao Weiguo, Guo Xijie, Wu Ping
Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, Jiangsu, China.
Key Laboratory of Silkworm and Mulberry Genetic Improvement, Ministry of Agriculture and Rural Affairs, Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu, China.
Arch Insect Biochem Physiol. 2020 Nov;105(3):e21735. doi: 10.1002/arch.21735. Epub 2020 Sep 2.
The disease caused by Bombyx mori nucleopolyhedrovirus (BmNPV) has always been difficult to control, resulting in tremendous economic losses in the sericulture industry. Although much has been learned about the impact of noncoding RNAs on pathogenesis, the role of circular RNA (circRNA) in insect immunity remains unclear. To explore circRNA regulation involved in BmNPV infection, we used transcriptome analysis of BmN cells with or without BmNPV infection to generate circRNA data set. A total of 444 novel circRNAs were identified in BmN cells, with 198 pervasively distributed both in the control group and BmNPV-infection group. The host genes were enriched inMAPK signaling pathway, dorso-ventral axis formation, and ECM-receptor interaction, which were required for the normal larval growth. A total of 75 circRNAs were differentially expressed (DE) on BmNPV infection. Six downregulated circRNAs were validated by Sanger sequencing and qRT-PCR. DEcircRNA-miRNA-DEmRNA network was constructed based on the six validated circRNAs. Pathway analysis indicated that the predicted target genes were mainly enriched in the metabolic pathway and immune-related signaling pathway. Our results may provide a basis for further studies on circRNA function in BmN cells challenged by BmNPV infection and offer an insight into the molecular mechanism on silkworm-virus interaction.
家蚕核型多角体病毒（BmNPV）引起的疾病一直难以控制，给养蚕业造成了巨大的经济损失。尽管人们已经对非编码RNA在发病机制中的作用有了很多了解，但环状RNA（circRNA）在昆虫免疫中的作用仍不清楚。为了探索circRNA在BmNPV感染中的调控作用，我们对感染或未感染BmNPV的BmN细胞进行转录组分析，以生成circRNA数据集。在BmN细胞中总共鉴定出444个新的circRNA，其中198个在对照组和BmNPV感染组中均广泛分布。宿主基因富集于丝裂原活化蛋白激酶（MAPK）信号通路、背腹轴形成和细胞外基质受体相互作用，这些都是幼虫正常生长所必需的。在BmNPV感染后，共有75个circRNA差异表达（DE）。通过桑格测序和qRT-PCR验证了6个下调的circRNA。基于这6个验证的circRNA构建了DEcircRNA-miRNA-DEmRNA网络。通路分析表明，预测的靶基因主要富集于代谢通路和免疫相关信号通路。我们的结果可能为进一步研究circRNA在受BmNPV感染的BmN细胞中的功能提供依据，并为深入了解家蚕与病毒相互作用的分子机制提供线索。
