Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies, Gyeonggi 17035, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Oct;70(10):5355-5362. doi: 10.1099/ijsem.0.004418. Epub 2020 Sep 3.
Two novel strains (HMF3257 and HMF4905), isolated from freshwater and bark samples, were investigated to determine their relationships within and between species of the genus by using a polyphasic approach. They were aerobic, Gram-stain-negative, non-motile and rod-shaped bacteria. The major fatty acids (>10%) in both strains were identified as summed feature 3 (C 7 and/or C 6) and C 5, while strains HMF3257 and HMF4905 contained a moderately high amount of C and iso-C, respectively. The predominant respiratory quinone was MK-7 for both strains. In addition to phosphatidylethanolamine and one unidentified glycolipid, the polar lipid profile of strain HMF3257 consisted of three unidentified aminophospholipids, one unidentified aminolipid and two unidentified polar lipids, and that of strain HMF4905 consisted of one unidentified aminophospholipid, two unidentified aminolipids and three unidentified polar lipids. The DNA G+C contents of strains HMF3257 and HMF4905 were 47.2 and 46.4 mol%, respectively. Phylogenetic analysis based on 16S rRNA gene sequences showed that strains HMF3257 and HMF4905 are closely related to 15J9-8 (97.0 % sequence similarity), while sharing 97.4 % sequence similarity with each other. The average nucleotide identity value between strains HMF3257 and HMF4905 was 81.1 %, and the digital DNA-DNA hybridization value between these two strains was 24.4 %. Based on the above data, strains HMF3257 and HMF4905 represent two novel members within the genus , for which the names sp. nov. and sp. nov. are proposed, respectively. The type strain of is HMF3257 (=KCTC 62463=NBRC 112670) and type strain of is HMF4905 (=KCTC 72779=NBRC 114270).
两株新菌株(HMF3257 和 HMF4905),分别从淡水和树皮样本中分离出来,通过多相研究来确定它们在属内和属间的关系。它们是需氧、革兰氏阴性、非运动和杆状细菌。两株菌的主要脂肪酸(>10%)均为 C7 和/或 C6 总和 C5,而菌株 HMF3257 和 HMF4905 分别含有相当数量的 C 和 iso-C。两株菌的主要呼吸醌均为 MK-7。除了磷脂酰乙醇胺和一种未鉴定的糖脂外,菌株 HMF3257 的极性脂谱由三种未鉴定的氨基磷脂、一种未鉴定的氨基脂和两种未鉴定的极性脂组成,而菌株 HMF4905 的极性脂谱由一种未鉴定的氨基磷脂、两种未鉴定的氨基脂和三种未鉴定的极性脂组成。菌株 HMF3257 和 HMF4905 的 DNA G+C 含量分别为 47.2 和 46.4 mol%。基于 16S rRNA 基因序列的系统发育分析表明,菌株 HMF3257 和 HMF4905 与 15J9-8 密切相关(97.0%序列相似性),彼此之间的序列相似性为 97.4%。菌株 HMF3257 和 HMF4905 之间的平均核苷酸同一性值为 81.1%,这两株菌之间的数字 DNA-DNA 杂交值为 24.4%。基于上述数据,菌株 HMF3257 和 HMF4905 代表属内的两个新成员,分别命名为 sp. nov.和 sp. nov.。的模式菌株为 HMF3257(=KCTC 62463=NBRC 112670), sp. nov.的模式菌株为 HMF4905(=KCTC 72779=NBRC 114270)。