Girbau M, Lesniak M A, Gomez J A, De Pablo F
Servicio de Endocrinologia, Hospital Sta. Cruz y S. Pablo, Barcelona, Spain.
Biochem Biophys Res Commun. 1988 May 31;153(1):142-8. doi: 10.1016/s0006-291x(88)81200-2.
Insulin receptors are present in chicken embryos at day 2 of development and insulin stimulates embryonic growth and differentiation. Most important, anti-insulin antibodies cause either death or developmental retardation in chicken embryos of that age. To determine if the embryo's endogenous insulin acts through its own receptor, we compared the effects of anti-insulin antibodies to the effects of anti-insulin receptor antibodies on growth and differentiation indexes in the chicken embryo. While the anti-insulin antibody caused a dose-dependent decrease in growth parameters like weight, total protein, DNA, RNA, total creatine kinase activity and a marker of differentiation, the creatine kinase-MB, the anti-insulin receptor antibody decreased all parameters except the creatine kinase-MB. Many, but not all, of the effects of insulin in early embryos, thus, are mediated through the insulin receptor.
胰岛素受体在鸡胚胎发育第2天就已存在，胰岛素可刺激胚胎生长和分化。最重要的是，抗胰岛素抗体可导致该年龄段鸡胚胎死亡或发育迟缓。为了确定胚胎内源性胰岛素是否通过自身受体发挥作用，我们比较了抗胰岛素抗体与抗胰岛素受体抗体对鸡胚胎生长和分化指标的影响。抗胰岛素抗体导致体重、总蛋白、DNA、RNA、总肌酸激酶活性以及分化标志物肌酸激酶-MB等生长参数呈剂量依赖性下降，而抗胰岛素受体抗体降低了除肌酸激酶-MB之外的所有参数。因此，胰岛素在早期胚胎中的许多（但并非全部）作用是通过胰岛素受体介导的。
