Lise D, Dubeaux C, Tello D, Mazier D, Jolivet M, Schlesinger D H, Audibert F, Chedid L
Department of Pharmacology and Therapeutics, University of South Florida, College of Medicine, Tampa 33612.
Biochem Biophys Res Commun. 1988 May 31;153(1):31-8. doi: 10.1016/s0006-291x(88)81185-9.
The immunogenicity of a peptide consisting of eight repeats of the tetrapeptide sequence NANP (Asn-Ala-Asn-Pro) contained in the circumsporozoite protein of Plasmodium falciparum was investigated in mice under different modes of presentation. This peptide was able to produce biologically active antibodies when administered with adjuvant and linked to a protein carrier. However, a (NANP) peptide polymerized by carbodiimide was found to be immunogenic in the absence of protein carrier in H-2b mice. In contrast, the (NANP)8 peptide polymerized by glutaraldehyde was not immunogenic in the same strain. Furthermore, the efficacy of murabutide in saline, as an immunological adjuvant, was compared to the efficacy of Freund's complete adjuvant.
在不同呈现方式下，对恶性疟原虫环子孢子蛋白中包含的由四肽序列NANP（天冬酰胺-丙氨酸-天冬酰胺-脯氨酸）的八个重复序列组成的肽的免疫原性进行了小鼠体内研究。该肽与佐剂一起给药并与蛋白质载体连接时能够产生生物活性抗体。然而，发现通过碳二亚胺聚合的（NANP）肽在H-2b小鼠中无蛋白质载体时具有免疫原性。相比之下，通过戊二醛聚合的（NANP）8肽在同一品系中没有免疫原性。此外，将盐水佐剂murabutide的功效与弗氏完全佐剂的功效进行了比较。
