Department of Molecular Biosystems, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya str., 35, Moscow 127276, Russian Federation.
Department of Molecular Biosystems, K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya str., 35, Moscow 127276, Russian Federation.
Gene. 2021 Jan 5;764:145055. doi: 10.1016/j.gene.2020.145055. Epub 2020 Sep 1.
Cyanobacteria are model photosynthetic prokaryotic organisms often used in biotechnology to produce biofuels including alcohols. The effect of alcohols on cyanobacterial cell physiology and specifically on membrane fluidity is poorly understood. Previous research on various primary aliphatic alcohols found that alcohols with a short hydrocarbon chain (C-C) do not affect expression of genes related to membrane physical state. In addition, less water-soluble alcohols with a hydrocarbon chain longer than C are found to have a reduced ability to reach cellular membranes hence do not drastically change membrane physical state or induce expression of stress-responsive genes. Therefore, hexan-1-ol (C) is suggested to have the most profound effect on cyanobacterial membrane physical state. Here, we studied the effects of hexan-1-ol on the cyanobacterium Synechocystis sp. PCC 6803 transcriptome. The transcriptome data obtained is compared to the previously reported analysis of gene expression induced by benzyl alcohol and butan-1-ol. The set of genes whose expression is induced after exposure to all three studied alcohols is identified. The expression under alcohol stress for several general stress response operons is analyzed, and examples of antisense interactions of RNA are investigated.
蓝藻是模式光合原核生物，常用于生物技术生产生物燃料，包括醇类。醇对蓝藻细胞生理学的影响，特别是对膜流动性的影响，知之甚少。先前对各种伯醇的研究发现，短链（C-C）烃的醇不会影响与膜物理状态相关的基因表达。此外，发现具有长于 C 的烃链的水溶性较低的醇到达细胞膜的能力降低，因此不会剧烈改变膜物理状态或诱导应激响应基因的表达。因此，己醇（C）被认为对蓝藻膜物理状态有最深远的影响。在这里，我们研究了己醇对蓝藻集胞藻 PCC 6803 转录组的影响。获得的转录组数据与先前报道的苄醇和丁醇诱导基因表达的分析进行了比较。确定了在暴露于所有三种研究醇类后表达诱导的基因集。分析了在酒精胁迫下几个一般应激反应操纵子的表达，并研究了 RNA 反义相互作用的实例。
