Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran.
Domest Anim Endocrinol. 2021 Jan;74:106486. doi: 10.1016/j.domaniend.2020.106486. Epub 2020 Apr 21.
Gonadotropin inhibitory hormone (GnIH), initially discovered in birds as a hypothalamic neuropeptide, inhibits the synthesis and release of gonadotropins by affecting GnRH neurons and gonadotropes. Therefore, it may be a key neuropeptide in reproduction in birds. The aim of the present study was to investigate the prepubertal, pubertal, and postpubertal localization of GnIH and changes in hypothalamic GnIH expression in British United Turkey hens. In prepubertal, pubertal, and postpubertal periods, the brains of turkey hens (n = 15) were removed after fixation. Sections (30 μm) were prepared from the entire hypothalamus and stained immunohistochemically against GnIH antibody. Gonadotropin inhibitory hormone-immunoreactive neurons were observed in the paraventricular nucleus. These neurons were significantly more abundant in the prepubertal turkeys than pubertal and postpubertal turkeys (P < 0.05). The results suggested that GnIH neurons have an important role in regulating the pubertal events in British United Turkey hens.
促性腺激素抑制激素（GnIH）最初在鸟类中作为下丘脑神经肽被发现，通过影响 GnRH 神经元和促性腺激素细胞来抑制促性腺激素的合成和释放。因此，它可能是鸟类生殖的关键神经肽。本研究旨在研究英国联合火鸡母鸡的GnIH 在青春期前、青春期和青春期后的定位以及下丘脑 GnIH 表达的变化。在青春期前、青春期和青春期后阶段，在固定后从火鸡母鸡（n = 15）的脑中取出大脑。从整个下丘脑制备 30μm 切片，并针对 GnIH 抗体进行免疫组织化学染色。在室旁核中观察到促性腺激素抑制激素免疫反应性神经元。这些神经元在青春期前的火鸡中明显比青春期和青春期后的火鸡中更为丰富（P < 0.05）。结果表明，GnIH 神经元在调节英国联合火鸡母鸡的青春期事件中具有重要作用。
