Department of Biological Sciences and Technology, School of Environmental Studies, China University of Geosciences, Wuhan 430074, China.
Aquatic Ecohealth Group, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
Int J Environ Res Public Health. 2020 Sep 1;17(17):6356. doi: 10.3390/ijerph17176356.
Harmful cyanobacterial blooms pose a risk to human health worldwide. To enhance understanding on the bloom-forming mechanism, the spatiotemporal changes in cyanobacterial diversity and composition in two eutrophic lakes (Erhai Lake and Lushui Reservoir) of China were investigated from 2010 to 2011 by high-throughput sequencing of environmental DNA. For each sample, 118 to 260 -IGS operational taxonomic units (OTUs) were obtained. Fifty-two abundant OTUs were identified, which made up 95.2% of the total sequences and were clustered into nine cyanobacterial groups. Although the cyanobacterial communities of both lakes were mainly dominated by , Erhai Lake had a higher cyanobacterial diversity. The abundance of mixed Nostocales species was lower than that of , whereas and were opportunistically dominant. The correlation between the occurrence frequency and relative abundance of OTUs was poorly fitted by the Sloan neutral model. Deterministic processes such as phosphorus availability were shown to have significant effects on the cyanobacterial community structure in Erhai Lake. In summary, the -dominated cyanobacterial community was mainly affected by the deterministic process. Opportunistically dominant species have the potential to replace and form blooms in eutrophic lakes, indicating the necessity to monitor these species for drinking water safety.
有害的蓝藻水华对全球人类健康构成威胁。为了深入了解水华形成机制，本研究采用高通量环境 DNA 测序技术，于 2010 年至 2011 年调查了中国两个富营养化湖泊（洱海和泸沽湖水库）中蓝藻多样性和组成的时空变化。每个样本获得了 118 到 260 个 16S rRNA 基因间隔区（IGS）操作分类单元（OTUs）。鉴定出 52 个丰富的 OTUs，占总序列的 95.2%，并聚类为 9 个蓝藻群。尽管两个湖泊的蓝藻群落主要由 组成，但洱海的蓝藻多样性更高。混合 Nostocales 物种的丰度低于 ，而 和 则是机会性优势种。OTUs 的出现频率和相对丰度之间的相关性与 Sloan 中性模型拟合不佳。确定性过程（如磷的可利用性）对洱海蓝藻群落结构有显著影响。总之，以 为主导的蓝藻群落主要受到确定性过程的影响。机会性优势种有可能取代 并在富营养化湖泊中形成水华，这表明有必要监测这些物种以保障饮用水安全。
