Masood S
Department of Pathology, University of Florida College of Medicine, Jacksonville.
Diagn Cytopathol. 1988 Mar;4(1):42-7. doi: 10.1002/dc.2840040111.
Steroid hormone receptors were studied in 45 patients with primary, recurrent, or metastatic ovarian cancer in cryostat-frozen sections and imprint preparations. The ligands, 17 B-estradiol-6-carboxymethyloxine-bovine serum albumin fluorescein isothiocyanate (FITC-BSA estradiol) and hydroxyprogesteronehemisuccinate bovine serum albumin tetramethylrhodamine isothiocyanate (TMRITC-BSA progesterone) were used in the fluorescent cytochemical method. Results were compared with standard dextran-coated charcoal (DCC) biochemical assay. An overall significant correlation between biochemical values and cytochemical results was found. However, the imprint results were more sensitive and more specific than the frozen section results. A statistically significant difference (P less than 0.05) was observed between touch preparation material and frozen section specimens by the fluorescent method.
在45例原发性、复发性或转移性卵巢癌患者的冷冻切片和印片标本中研究了类固醇激素受体。采用荧光细胞化学方法，使用配体17β-雌二醇-6-羧甲基肟-牛血清白蛋白异硫氰酸荧光素（FITC-BSA雌二醇）和羟基孕酮半琥珀酸牛血清白蛋白四甲基罗丹明异硫氰酸酯（TMRITC-BSA孕酮）。将结果与标准葡聚糖包被活性炭（DCC）生化分析进行比较。发现生化值与细胞化学结果之间总体存在显著相关性。然而，印片结果比冷冻切片结果更敏感、更具特异性。通过荧光法观察到，触摸制片材料与冷冻切片标本之间存在统计学显著差异（P小于0.05）。
