Department of Pathophysiology of Hearing and Balance, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University, Toruń, Poland.
Multidisciplinary City Hospital, Rehabilitation Center, Bydgoszcz, Poland.
Oxid Med Cell Longev. 2020 Aug 20;2020:9792836. doi: 10.1155/2020/9792836. eCollection 2020.
Deep electromagnetic stimulation (DEMS) and low-frequency ultrasound (US) are new physical therapy methods used in the rehabilitation of the musculoskeletal system and wound healing. They are applied locally to treat the injured tissues. The beneficial effects of these methods in supportive care have been documented, but accurate biochemical effects are not known. The goal was to assess the effect of single DEMS and US sessions on the oxidant-antioxidant equilibrium, as well as the activities of lysosomal hydrolases and -antitrypsin (AAT) in peripheral blood of juvenile injured amateur athletes. In the athletes with low back pain (DEMS treated, = 16) and pain in the shoulder or ankle joint (US treated, = 14), as well as in healthy control amateur athletes (DEMS treated, = 14; US treated, = 17), before the sessions and 30 minutes and 24 hours after them, the levels of the following parameters were determined: thiobarbituric acid reactive substances (TBARS) in erythrocytes and plasma, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) in erythrocytes, as well as acid phosphatase (AcP), arylsulfatase (ASA), cathepsin D (CTS D), and -antitrypsin (AAT) in serum. After both procedures, the levels of parameters changed in a negligible manner, excluding the cathepsin D activity, which was statistically significantly lower 30 min and 24 h after US in the control athletes compared to the baseline activity determined directly before the procedure (47.5% and 55.7% differences, respectively). Similar tendency was observed after DEMS ( > 0.05). The procedures, especially low-frequency US, decrease lysosomal proteolytic activity and do not significantly disrupt the oxidant-antioxidant and lysosomal equilibriums in the peripheral blood both of healthy and injured athletes. No systemic acute-phase response of AAT was also detected in the athletes after both procedures. This trial is registered with CTRI/2018/01/011344.
深部电磁刺激（DEMS）和低频超声（US）是用于肌肉骨骼系统康复和伤口愈合的新物理治疗方法。它们局部应用于治疗受伤组织。这些方法在支持性护理中的有益效果已经得到证实，但确切的生化作用尚不清楚。目的是评估单次 DEMS 和 US 治疗对氧化还原平衡以及周围血液中溶酶体水解酶和 -抗胰蛋白酶（AAT）活性的影响。在患有腰痛（DEMS 治疗，n = 16）和肩部或踝关节疼痛（US 治疗，n = 14）的青少年受伤业余运动员以及健康对照组业余运动员（DEMS 治疗，n = 14；US 治疗，n = 17）中，在治疗前和治疗后 30 分钟和 24 小时，测定以下参数的水平：红细胞和血浆中的硫代巴比妥酸反应物质（TBARS）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）以及血清中的酸性磷酸酶（AcP）、芳基硫酸酯酶（ASA）、组织蛋白酶 D（CTS D）和 -抗胰蛋白酶（AAT）。两种方法后，参数水平变化微不足道，Cathepsin D 活性除外，与直接在治疗前测定的基础活性相比，US 治疗后 30 分钟和 24 小时的活性分别降低 47.5%和 55.7%（差异有统计学意义）。在 DEMS 后也观察到类似的趋势（> 0.05）。这些方法，特别是低频 US，降低了溶酶体蛋白水解活性，并且在健康和受伤运动员的外周血液中，氧化还原和溶酶体平衡均未受到明显干扰。两种方法后，运动员中也未检测到 AAT 的全身急性期反应。该试验已在 CTRI/2018/01/011344 注册。
