Dahl S C, Grabel L B
Department of Biology, Wesleyan University, Middletown, Connecticut 06457.
Exp Cell Res. 1988 Jun;176(2):234-47. doi: 10.1016/0014-4827(88)90327-8.
F-9 teratocarcinoma stem cells differentiate into parietal endoderm when monolayer cultures are treated with retinoic acid. This change in phenotype is accompanied by increased accumulation and altered organization of fibronectin deposits. Although both stem cells and treated cells synthesize and accumulate fibronectin, only the treated cells deposit a fibrillar array of the protein. We have monitored the accumulation of fibronectin in nontreated and treated F-9 cells with indirect immunofluorescence and have biochemically analyzed the fibronectin synthesized by each cell type with one- and two-dimensional acrylamide gels and peptide maps. Our data suggest that no differences exist between these fibronectins to account for the observed changes in accumulation. Thus, another mechanism may regulate the organization of matrix deposition.
当单层培养的F-9畸胎癌细胞用视黄酸处理时，它们会分化为滋养层内胚层。这种表型变化伴随着纤连蛋白沉积物积累的增加和组织方式的改变。虽然干细胞和处理后的细胞都能合成并积累纤连蛋白，但只有处理后的细胞会沉积出该蛋白的纤维状阵列。我们用间接免疫荧光监测了未处理和处理后的F-9细胞中纤连蛋白的积累情况，并用一维及二维丙烯酰胺凝胶和肽图对每种细胞类型合成的纤连蛋白进行了生化分析。我们的数据表明，这些纤连蛋白之间不存在差异来解释观察到的积累变化。因此，可能有另一种机制调节基质沉积的组织方式。
