从鲁西斗鸡中分离到的两种新型重组禽白血病病毒。

Two novel recombinant avian leukosis virus isolates from Luxi gamecock chickens.

机构信息

Institute of Microbe and Host Health, Linyi University, Linyi, 276005, Shandong, China.

出版信息

Arch Virol. 2020 Dec;165(12):2877-2881. doi: 10.1007/s00705-020-04799-w. Epub 2020 Sep 3.

Abstract

Avian leukosis virus (ALV) is associated with immune suppression, neoplasia, and reduced performance in chickens. In this study, two strains of ALV were isolated from Luxi gamecocks by DF-1 cell culture and identified by PCR, immunofluorescence assay, and sequencing of the viral genome. These strains were found to be novel recombinant viruses with nucleotide sequence identity of over 93.0% in the LTR and 94.4% in U3 to ALV-J, over 95.0% in the 5'UTR to ALV-C, over 93.4% in gp85 to ALV-B, and over 96.0% in gp37 to ALV-E. These results indicate that these two isolates are recombinants between ALV-J, ALV-C, ALV-E and ALV-B.

摘要

禽白血病病毒（ALV）与鸡的免疫抑制、肿瘤形成和生产性能下降有关。本研究通过 DF-1 细胞培养从鲁西斗鸡中分离到两株 ALV，并通过 PCR、免疫荧光检测和病毒基因组测序进行鉴定。这两种毒株被鉴定为新型重组病毒，其 LTR 区核苷酸序列同源性超过 93.0%，与 ALV-J 同源；U3 区核苷酸序列同源性超过 94.4%，与 ALV-J 同源；5'UTR 区核苷酸序列同源性超过 95.0%，与 ALV-C 同源；gp85 区核苷酸序列同源性超过 93.4%，与 ALV-B 同源；gp37 区核苷酸序列同源性超过 96.0%，与 ALV-E 同源。这些结果表明，这两种分离株是 ALV-J、ALV-C、ALV-E 和 ALV-B 之间的重组病毒。