一种用于选择性检测脂质焦磷酸的三联吡啶锌配合物：用于监测细菌 O-和 N-转糖基化的模型体系。
A Terpyridine Zinc Complex for Selective Detection of Lipid Pyrophosphates: A Model System for Monitoring Bacterial O- and N-Transglycosylations.
机构信息
Department of Chemistry, National Taiwan University, Taipei 106, Taiwan.
The Genomics Research Center, Academia Sinica, Taipei 115, Taiwan.
出版信息
J Org Chem. 2020 Oct 2;85(19):12747-12753. doi: 10.1021/acs.joc.0c01252. Epub 2020 Sep 18.
To develop an effective method for probing O- and N-glycosyltransfer reactions that are accompanied by the release of undecaprenyl pyrophosphate, solanesyl pyrophosphate (SPP) is used as a surrogate to bind a terpyridine zinc complex (Tpy-Zn), forming a fluorescent [Tpy-Zn]-SPP complex ( 106,000 M in EtOH-CHCl) with 5.8 μM LOD in HEPES buffer (10 mM, pH 7.4) containing 10 mM CaCl and 0.08% decyl PEG, which is similar to the bioassay conditions for lipid II polymerization.
为了开发一种有效探测伴随释放焦磷酸未酰基载体蛋白的 O-和 N-糖基转移反应的方法，使用法呢基焦磷酸（SPP）作为取代物与三联吡啶锌配合物（Tpy-Zn）结合，形成一种在乙醇-氯仿中具有 106,000 M 的荧光 [Tpy-Zn]-SPP 配合物（LOD 为 5.8 μM），在含有 10 mM CaCl 和 0.08% 十二烷基聚乙二醇的 HEPES 缓冲液（10 mM，pH 7.4）中，其与脂质 II 聚合的生物测定条件相似。
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