CAS Key Laboratory of Soft Matter Chemistry, Department of Chemistry, University of Science and Technology of China , Hefei, Anhui 230026, China.
Anal Chem. 2015 Mar 3;87(5):2678-84. doi: 10.1021/ac504536q. Epub 2015 Feb 23.
Herein, we report a new pyridine-biquinoline-derivative fluorophore L for effectively sensing pyrophosphate (PPi) and monohydrogen sulfide (HS(-)) in aqueous buffer and in living cells. L could selectively coordinate with metal ions (M(n+)) in Groups IB and IIB to form L-M(n+) complexes with 1:1 stoichiometry, resulting in fluorescence quenching via photoinduced electron transfer (PET) mechanism. L-Zn(2+) complex was applied to competitively coordinate with PPi to form a new "ate"-type complex, turning on the fluorescence by a 21-fold-increase. The limit of detection (LOD) of this assay for PPi detection in aqueous buffer is 0.85 μM. L-Cu(2+) complex was applied for highly selective detection of HS(-) with an excellent sensitivity by 25-fold decomplexation-induced fluorescence increase. LOD of L-Cu(2+) complex for HS(-) detection in aqueous buffer is 2.24 μM. With the in vitro data obtained, we successfully applied these two complexes for sequential imaging Zn(2+) and PPi, Cu(2+) and HS(-) in living cells, respectively. Since PPi and HS(-) occur in vascular calcification in positive correlation, our multifunctional probe L might help doctors to more precisely diagnose this disease in vivo in the future. For example, we could use radioactive tracer L-(64)Cu for qualitative and quantitative positron emission tomography/computed tomography (PET/CT) imaging of HS(-) in vivo.
在此，我们报告了一种新的吡啶-双喹啉衍生荧光团 L，可有效感应焦磷酸盐 (PPi) 和一价氢硫化物 (HS(-)) 在水缓冲液和活细胞中。L 可以选择性地与 IB 族和 IIB 族的金属离子 (M(n+)) 配位，形成 1:1 化学计量比的 L-M(n+) 配合物，通过光诱导电子转移 (PET) 机制导致荧光猝灭。L-Zn(2+) 配合物被应用于与 PPi 竞争配位，形成新的“内盐”型配合物，通过 21 倍的荧光增强来开启荧光。该测定法在水缓冲液中检测 PPi 的检测限 (LOD) 为 0.85 μM。L-Cu(2+) 配合物通过 25 倍的解络合诱导荧光增强，对 HS(-) 具有优异的选择性和高灵敏度检测。L-Cu(2+) 配合物在水缓冲液中检测 HS(-)的 LOD 为 2.24 μM。根据体外数据，我们成功地将这两种配合物分别用于活细胞中 Zn(2+) 和 PPi、Cu(2+) 和 HS(-) 的顺序成像。由于 PPi 和 HS(-) 在血管钙化中呈正相关，我们的多功能探针 L 将来可能有助于医生更精确地在体内诊断这种疾病。例如，我们可以使用放射性示踪剂 L-(64)Cu 对 HS(-)进行定性和定量的正电子发射断层扫描/计算机断层扫描 (PET/CT) 成像。
