Sartore Douglas Morisue, Vargas Medina Deyber Arley, Costa Jose Luiz, Lanças Fernando Mauro, Santos-Neto Álvaro José
São Carlos Institute of Chemistry, University of São Paulo, 13566-590, São Carlos, SP, Brazil.
Faculty of Pharmaceutical Sciences, University of Campinas, 13083-859, Campinas, SP, Brazil.
Talanta. 2020 Nov 1;219:121185. doi: 10.1016/j.talanta.2020.121185. Epub 2020 May 21.
An original, selective and automated method, for the microextraction by packed sorbent (MEPS) of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (THC-OH), and 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) from human urine, was developed by using (i) a catechin-molded molecularly imprinted polymer (MIP), (ii) a new lab-made MEPS device easily repackable with any commercial or lab-made sorbent, and (iii) a lab-made multi syringe autosampler. Analyses were performed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and the developed method proved to be precise, accurate and showed good linearity. Determination coefficients ranged from 0.96 to 0.99, in the range of 5-250 ng mL. Limits of detection and quantification ranged between 1.0 and 5.0 ng mL and 5.0 and 20.0 ng mL. The method was successfully applied in the analysis of real urine samples. The same packed syringe was effectively used over 90 consecutive extractions without carry-over effects.
通过使用（i）儿茶素成型的分子印迹聚合物（MIP）、（ii）一种易于用任何市售或自制吸附剂重新装填的新型自制微萃取填充吸附剂（MEPS）装置以及（iii）一种自制的多注射器自动进样器，开发了一种从人尿液中对Δ9 - 四氢大麻酚（THC）、11 - 羟基 - Δ9 - 四氢大麻酚（THC - OH）和11 - 去甲 - Δ9 - 四氢大麻酚 - 9 - 羧酸（THC - COOH）进行微萃取的原创、选择性和自动化方法。通过液相色谱 - 串联质谱（LC - MS/MS）进行分析，所开发的方法被证明是精确、准确的，并且具有良好的线性。在5 - 250 ng/mL范围内，测定系数在0.96至0.99之间。检测限和定量限分别在1.0至5.0 ng/mL和5.0至20.0 ng/mL之间。该方法成功应用于实际尿液样本的分析。同一支填充注射器在连续90多次萃取中有效使用，没有残留效应。
