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过敏炎症改变大鼠脂肪组织中的 microRNA 表达谱。

Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat.

机构信息

Department of Animal Physiology, Biochemistry and Biostructure, Poznań University of Life Sciences, 60-637 Poznań, Poland.

Molecular and Cell Biology Unit, Department of Pediatric Pulmonology, Allergy and Clinical Immunology, Poznan University of Medical Sciences, 60-572 Poznań, Poland.

出版信息

Genes (Basel). 2020 Sep 2;11(9):1034. doi: 10.3390/genes11091034.

DOI:10.3390/genes11091034
PMID:32887419
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7564923/
Abstract

Adipose tissue is a major source of circulating exosomal microRNAs (miRNAs) that are modulators of the immune response in various types of tissues and organs, including airways. Still, no evidence exists if allergic airway inflammation may affect fat tissue inflammation via alterations in the miRNA expression profile. Therefore, we investigated the miRNA expression profile in the adipose tissue upon induced allergic inflammation in the airways in the rat. Brown Norway rats were chronically sensitized to house dust mite extract for seven weeks. Body composition was performed using MiniSpec Plus. The eosinophil count and the total IgE level were determined to confirm the induction of allergic inflammation. MiRNA expression profiling was done using the next-generation sequencing with validation by qPCR. We found that allergic airway inflammation significantly increased fat in adipose tissue, glucose concentration, and the gene expression of adipose tissue-derived proinflammatory peptides (leptin, TNFα). In miRNA-seq analysis, we showed significant differences in the expression of 36 mature miRNAs, three precursors, and two miRNA families in adipose tissue of allergic rats. Two miRNAs-miRNA-151-5p and miRNA-423-3p-showed significantly increased expression in qPCR in adipose tissue and lungs of sensitized animals. Allergic airway inflammation affects fat tissue and alters miRNA expression profile in adipose tissue in the rat.

摘要

脂肪组织是循环外泌体 microRNAs（miRNAs）的主要来源，miRNAs 是各种组织和器官（包括气道）免疫反应的调节剂。然而，目前尚无证据表明过敏性气道炎症是否可以通过改变 miRNA 表达谱来影响脂肪组织炎症。因此，我们研究了在大鼠气道中诱导过敏性炎症时脂肪组织中的 miRNA 表达谱。褐鼠被慢性致敏于屋尘螨提取物 7 周。使用 MiniSpec Plus 进行体成分分析。通过确定嗜酸性粒细胞计数和总 IgE 水平来确认过敏性炎症的诱导。使用下一代测序进行 miRNA 表达谱分析，并通过 qPCR 进行验证。我们发现，过敏性气道炎症显著增加了脂肪组织中的脂肪、葡萄糖浓度以及脂肪组织衍生的促炎肽（瘦素、TNFα）的基因表达。在 miRNA-seq 分析中，我们显示了过敏性大鼠脂肪组织中 36 个成熟 miRNA、3 个前体和 2 个 miRNA 家族的表达存在显著差异。在 qPCR 中，两种 miRNA-miRNA-151-5p 和 miRNA-423-3p-在致敏动物的脂肪组织和肺部中显示出显著增加的表达。过敏性气道炎症会影响脂肪组织，并改变大鼠脂肪组织中的 miRNA 表达谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/215042566b13/genes-11-01034-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/35f4657eb9ad/genes-11-01034-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/a612bbd90cc7/genes-11-01034-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/852be32487dc/genes-11-01034-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/65df0b084352/genes-11-01034-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/3e07da87ac68/genes-11-01034-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/2485a8443e48/genes-11-01034-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/215042566b13/genes-11-01034-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/35f4657eb9ad/genes-11-01034-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/a612bbd90cc7/genes-11-01034-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/852be32487dc/genes-11-01034-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/65df0b084352/genes-11-01034-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/3e07da87ac68/genes-11-01034-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/2485a8443e48/genes-11-01034-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/223d/7564923/215042566b13/genes-11-01034-g007.jpg

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